dc.creator | Mihailović, Vladimir | |
dc.creator | Mišić, Danijela | |
dc.creator | Matić, Sanja | |
dc.creator | Mihailović, Mirjana | |
dc.creator | Stanić, Snežana | |
dc.creator | Vrvić, Miroslav M. | |
dc.creator | Katanić, Jelena | |
dc.creator | Mladenović, Milan | |
dc.creator | Stanković, Nevena | |
dc.creator | Boroja, Tatjana | |
dc.creator | Stanković, Milan S. | |
dc.date.accessioned | 2016-05-23T11:00:55Z | |
dc.date.issued | 2015 | |
dc.identifier.issn | 0926-6690 | |
dc.identifier.uri | https://radar.ibiss.bg.ac.rs/handle/123456789/2350 | |
dc.description.abstract | The purpose of this study was to evaluate the antioxidant potential of
methanol extracts of Gentiana crudata L. aerial parts and roots, as well
as the stability of the phenolic compounds and antioxidant capacity of
extracts during heating, at different pHs and after an in vitro
digestion procedure. Also, their genotoxicity and antigenotoxicity
against carbon tetrachloride in the liver of albino Wistar rats using
the comet assay were evaluated. Three secoiridoid glycosides
(swertiamarin, gentiopicrin, and sweroside) and four phenolic compounds
(orientin, vitexin and two isovitexin-glucosides) were identified as the
major constituents in aerial parts and roots of G. cruciata, using
UHPLC-DAD/+/- HESI-MS/MS analysis. The results of antioxidant assays
showed that aerial parts displayed higher antioxidant activity compared
to the roots, which could be related to higher phenolics content,
especially flavonoids. In general, extracts showed pH and thermal
stability, while duodenal condition had more influence on total phenolic
condition and antioxidant activity of extracts. Both extracts showed a
protective effect against CCl4 in comet assays. The roots extract showed
no genotoxic activity, while aerial parts extract showed slight
genotoxicity at concentrations of 400 mg/kg b.w. (C) 2015 Elsevier B.V.
All rights reserved. | en |
dc.description.sponsorship | Ministry of Education, Science and Technological Development of the
Republic of Serbia {[}III 43004, III 41010, OI 173024] | |
dc.language | English | |
dc.publisher | Elsevier | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/43004/RS// | |
dc.relation | info:eu-repo/grantAgreement/MESTD/MPN2006-2010/141010/RS// | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173024/RS// | |
dc.rights | restrictedAccess | |
dc.source | Industrial Crops and Products | |
dc.subject | Gentiana cruciata L. | |
dc.subject | Antioxidant activity | |
dc.subject | Genotoxicity | |
dc.subject | Antigenotoxicity | |
dc.subject | Extract stability | |
dc.subject | In vitro digestion | |
dc.title | Comparative phytochemical analysis of Gentiana cruciata L. roots and
aerial parts, and their biological activities | en |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Врвиц, Мирослав М.; Катаниц, Јелена; Станковиц, Милан С.; Станиц, Снезана; Матиц, Сања; Мисиц, Данијела; Михаиловиц, Владимир; Бороја, Татјана; Михаиловић, Мирјана В.; Станковиц, Невена; Младеновиц, Милан; | |
dc.rights.holder | © 2015 Elsevier B.V. | |
dc.citation.volume | 73 | |
dc.identifier.doi | 10.1016/j.indcrop.2015.04.013 | |
dc.identifier.scopus | 2-s2.0-84928531344 | |
dc.identifier.wos | 000357839300006 | |
dc.citation.spage | 49 | |
dc.citation.epage | 62 | |
dc.type.version | publishedVersion | en |
dc.citation.rank | aM21 | |