Comparative phytochemical analysis of Gentiana cruciata L. roots and aerial parts, and their biological activities
2015
Аутори:
Mihailović, VladimirMišić, Danijela
Matić, Sanja
Mihailović, Mirjana
Stanić, Snežana
Vrvić, Miroslav M.
Katanić, Jelena
Mladenović, Milan
Stanković, Nevena
Boroja, Tatjana
Stanković, Milan S.
Тип документа:
Чланак у часопису (Објављена верзија)
,
© 2015 Elsevier B.V.
Метаподаци
Приказ свих података о документуАпстракт:
The purpose of this study was to evaluate the antioxidant potential of
methanol extracts of Gentiana crudata L. aerial parts and roots, as well
as the stability of the phenolic compounds and antioxidant capacity of
extracts during heating, at different pHs and after an in vitro
digestion procedure. Also, their genotoxicity and antigenotoxicity
against carbon tetrachloride in the liver of albino Wistar rats using
the comet assay were evaluated. Three secoiridoid glycosides
(swertiamarin, gentiopicrin, and sweroside) and four phenolic compounds
(orientin, vitexin and two isovitexin-glucosides) were identified as the
major constituents in aerial parts and roots of G. cruciata, using
UHPLC-DAD/+/- HESI-MS/MS analysis. The results of antioxidant assays
showed that aerial parts displayed higher antioxidant activity compared
to the roots, which could be related to higher phenolics content,
especially flavonoids. In general, extracts showed pH and thermal
stability, while duodenal condition had more influence on total phenolic
condition and antioxidant activity of extracts. Both extracts showed a
protective effect against CCl4 in comet assays. The roots extract showed
no genotoxic activity, while aerial parts extract showed slight
genotoxicity at concentrations of 400 mg/kg b.w. (C) 2015 Elsevier B.V.
All rights reserved.
Кључне речи:
Gentiana cruciata L.; Antioxidant activity; Genotoxicity; Antigenotoxicity; Extract stability; In vitro digestionИзвор:
Industrial Crops and Products, 2015, 73, 49-62Финансирање / пројекти:
- Симултана биоремедијација и соилификација деградираних простора, за очување природних ресурса биолошки активних супстанци и развој и производњу биоматеријала и дијететских производа (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-43004)
- Одређивање атомских параметара на основу облика спектралних линија (RS-MESTD-MPN2006-2010-141010)
- Физиолошка, хемијска и молекуларна анализа диверзитета одабраних ретких и угрожених биљних врста у циљу еx ситу заштите и продукције биолошки активних једињења (RS-MESTD-Basic Research (BR or ON)-173024)
DOI: 10.1016/j.indcrop.2015.04.013
ISSN: 0926-6690