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Karakterizacija tripsinima-sličnih enzima srednjeg creva larvi Morimus funereus (Coleoptera: Cerambycidae)
Characterization of trypsin-like enzymes from the midgut of Morimus funereus (Coleoptera: Cerambycidae) larvae
dc.creator | Lončar, N. | |
dc.creator | Božić, Nataša | |
dc.creator | Nenadović, Vera | |
dc.creator | Ivanović, Jelisaveta | |
dc.creator | Vujčić, Zoran M. | |
dc.date.accessioned | 2015-08-28T10:26:51Z | |
dc.date.available | 2015-08-28T10:26:51Z | |
dc.date.issued | 2009 | sr |
dc.date.issued | 2009 | |
dc.identifier.issn | 0354-4664 | sr |
dc.identifier.other | Rad_konverzija_303 | sr |
dc.identifier.uri | https://radar.ibiss.bg.ac.rs/handle/123456789/238 | |
dc.description.abstract | The pH along the midgut of M. funereus larvae had different values, being acidic in the anterior section and basic in the middle and posterior sections. Elastase- and chymotrypsin-like activities were highest in the middle, low in the anterior, and negligible in the posterior section of the midgut. Trypsin-like activities were detected along the whole midgut, with more than 90% of activity in the anterior section. The level of elastase- and chymotrypsin-like activity was very low compared to trypsin-like activity. In the anterior section of the midgut, two isoforms of trypsin-like enzymes were found, both being basic and almost completely inhibited by benzamidine. | en |
dc.description.abstract | pH vrednost duž srednjeg creva larvi M. funereus se razlikuje, kisela je u regionu prednjeg dela, dok je u srednjem i zadnjem delu bazna. Elastazama i himotripsinima slična aktivnost je najveća u srednjem delu srednjeg creva dok je u prednjem delu detektovana mala vrednost, a u zadnjem delu zanemarljiva. Tripsinima slična aktivnost je detektovana duž celog srednjeg creva, s tim da se više od 90 % aktivnosti detektuje u prednjem delu srednjeg creva. Zastupljenost elastazama i himotripsinima sličnih endopeptidaza je zanemarljivo mala u poređenju sa zastupljenošću tripsinima sličnih enzima. U prednjem delu srednjeg creva nalaze se dve izoforme tripsinima sličnih enzima, sa baznim pI vrednostima, koje su skoro u potpunosti inhibirane benzamidinom. | sr |
dc.description.sponsorship | Projekat ministarstva br. 142026B | sr |
dc.language.iso | eng | sr |
dc.rights | openAccess | sr |
dc.source | Archives of Biological Sciences | sr |
dc.subject | Cerambycidae | ENG |
dc.subject | Morimus funereus | ENG |
dc.subject | trypsin | ENG |
dc.subject | midgut peptidases | ENG |
dc.subject | synthetic substrate | ENG |
dc.subject | zymogram | ENG |
dc.title | Karakterizacija tripsinima-sličnih enzima srednjeg creva larvi Morimus funereus (Coleoptera: Cerambycidae) | sr |
dc.title | Characterization of trypsin-like enzymes from the midgut of Morimus funereus (Coleoptera: Cerambycidae) larvae | en |
dc.type | article | sr |
dc.rights.license | ARR | |
dcterms.abstract | Вујчић, Зоран М.; Ненадовић, Вера; Ивановић, Јелисавета; Божић, Наташа; Лончар, Н.; | |
dc.citation.issue | 4 | sr |
dc.citation.volume | 61 | sr |
dc.citation.spage | 713 | sr |
dc.citation.epage | 718 | sr |
dc.type.version | publishedVersion | sr |
dc.identifier.fulltext | https://radar.ibiss.bg.ac.rs//bitstream/id/2129/Rad_konverzija_303.pdf | |
dc.citation.rank | M23 | |
dc.identifier.rcub | https://hdl.handle.net/21.15107/rcub_ibiss_238 |