Antioksidativno, antimikrobno i alelopatsko dejstvo tri endemične vrste roda Nepeta (Lamiaceae)
Antioxidative, antimicrobial and allelopathic effects of three endemic Nepeta species (Lamiaceae)
2013
Authors:
Nestorović Živković, JasminaContributors
Mišić, DanijelaSabovljević, Aneta
Živković, Suzana
Ćirić, Ana
Document Type:
Doctoral thesis (Published version)
Metadata
Show full item recordAbstract:
Nepeta rtanjnsis Diklić and Milojević, N. sibirica L. and N. nervosa Royle ex
Bentham (fam. Lamiaceae) are endemic species of genus Nepeta that are insufficiently
phytochemically characterized and poorly tested in a sense of their biological activity.
These three species are characterized by different qualitative and quantitative
nepetalactone content, what makes them ideal objects in investigating the biological
activity of different stereoisomers of this compound. Considering that these species are
rare, and extremely endangered in case of N. rtanjensis, plant material for the
experiments was obtained by micropropagation. The research included phytochemical
characterization of in vitro grown three Nepeta species , as well as analysis of their
bioactivities such as antimicrobial, antioxidative activity and allelopathic potential.
Phytochemical characterization of three Nepeta species grown in vitro, involving
GC-MS, GC-FID, PTR-MS, Headspace GC-MS, HPLC-UV, HPLC-MS, and
UHPLC/DAD/±HESI-MS/MS analyses, enabled identification and quantification of the
main secondary metabolites (terpenes and phenolics). The dominant volatile compound
from terpenes group is monoterpene lacton - nepetalactone, whereby its trans,cisisomer
is mostly present in N. rtanjensis and its cis,trans- isomer in N. sibirica. In
N. nervosa, nepetalactone has been detected only in traces. In all investigated species,
rosmarinic acid is a dominant phenolic compound, while the other phenolic acids
(chlorogenic, neochlorogenic and caffeic acids) are present in significantly lower
concentrations.
The results of ABTS and DPPH assays showed that methanol extracts of
N. rtanjensis, N. sibirica and especially N. nervosa, possess considerable antioxidant
activities, and the FRAP assay revealed high ferric reducing capacity for all the samples
tested.. Significant antioxidant activity is attributed to phenolic acids, in the first place
to rosmarinic acid.
Nepetalactone doesn’t contribute notably to the antioxidant potential of the methanol
extracts, but there is a possibility of antagonistic action between phenolic acids and
nepetalactone. The methanol extracts of three Nepeta species show significant
antimicrobial activity against eight bacterial and eight fungal species, and the activity
originates from monoterpenic lactones and phenolic acids. The highest antimicrobial
activity was recorded for N. rtanjensis methanol extract, while extracts of N. sibirica
and N. nervosa were less efficient. Nepetalactone stereochemistry substantially
determines its antimicrobial activity, whereby the trans,cis- isomer is more active than
the cis,trans- one.
It has been confirmed that N. rtanjensis and N. sibirica possess compounds that
can influence growth and development of other plant species in their proximate
surrounding, thus actively participating in allelopathic interactions. Allelopathic effect
of N. rtanjensis and N. sibirica originates from the dominant bioactive compound of
these species - nepetalactone. Stereochemistry of nepetalactone considerably determines
its allelopathic potential, with trans,cis- isomer being more active than the cis,transnepetalactone.
Allelopathic effect of nepetalactone is reflected through slugged dynamic
of seed germination in the test species Lepidium sativum L., but also through its effect
on biochemical processes that are the consequence of plant’s disturbed antioxidative
system. Allelopathic effect of nepetalactone, especially of its trans,cis- isomer, leads to
the changes in antioxidative system of cress seedlings by violating normal models of
antioxidative enzymes expression and activity (POD, CAT, SOD, PPO). In cress
seedlings, the inhibition of activities and changed profiles of POD, CAT, Fe-SOD and
CuZn-SOD isoforms were observed. Trans,cis- nepetalactone action leads to the
increased content of total phenols in the first phases of growth and development of
L. sativum seedlings, which is followed by earlier induction of expression and increased
PPO activity. During allelopathic interactions, nepetalactone induces certain
modifications of sinapate metabolism in cress seedlings, which in general can be
described by decreased conversion of sinapoylholin to sinapoylglucose, and further to
sinapoylmalate.
Allelopathic features of N. rtanjensis and N. sibirica indicate the possibility of
successful application of these species and their bioactive components in agricultural
practice, as bioherbicides, toward weed control. In this thesis the allelopathic effect of
N. rtanjensis and N. sibirica was confirmed in case of crops L. sativum, Lactuca sativa
L. cv. „Majska kraljica“, Lotus corniculatus L. cv. „Bokor“, and Brassica napus L., and
weeds Stellaria media (L.) Vill., Rumex crispus L., and Arabidopsis thaliana (L.)
Heynh. ecotype Colombia.
Keywords:
Nepeta rtanjensis; Nepeta sibirica; Nepeta nervosa; Nepetalactone; Phenolic acid; Antioxidative potential; Antimicrobial effects; Allelopathy; Antioxidant enzymeSource:
University of Belgrade, Faculty of Biology, 2013, 1-218Funding / projects:
- Physiological, chemical and molecular analysis of the diversity of selected rare and endangered plant species and application of biotechnology for ex situ conservation and production of biologically active compounds (RS-MESTD-Basic Research (BR or ON)-173024)
URI
http://eteze.bg.ac.rs/application/showtheses?thesesId=790https://fedorabg.bg.ac.rs/fedora/get/o:7112/bdef:Content/download
http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=44652047
http://nardus.mpn.gov.rs/123456789/2132
https://radar.ibiss.bg.ac.rs/handle/123456789/2401
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IBISSAPA:
Nestorović-Živković, J. (2013). Antioksidativno, antimikrobno i alelopatsko dejstvo tri endemične vrste roda Nepeta (Lamiaceae). University of Belgrade, Faculty of Biology. p. 218.Vancouver:
Nestorović-Živković J. Antioksidativno, antimikrobno i alelopatsko dejstvo tri endemične vrste roda Nepeta (Lamiaceae) [dissertation]. Belgrade: University of Belgrade, Faculty of Biology; 2013. 218 p.Related items
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