Uticaj elektromagnetnog polja (2 mT, 50 Hz) na spontanu bioelektričnu aktivnost neurona antenalnog lobusa adulta Morimus funereus (Insecta, Coleoptera)
The influence of electromagnetic field (2 mT, 50 Hz) on spontaneous bioelectrical activity of antennal lobe neurons of adult Morimus funereus (Insecta, Coleoptera)
2013
Download 🢃
Authors:
Todorović, DajanaContributors
Kalauzi, AleksandarŽivić, Miroslav
Prolić, Zlatko
Tomanović, Željko
Nikolić, Ljiljana
Document Type:
Doctoral thesis (Published version)
Metadata
Show full item recordAbstract:
External magnetic fields (MF) disturb the equilibrium of biological systems, even
during short-term exposure, resulting in disturbances at different levels of organization.
These changes accumulate in organisms, which is one of the reasons for testing biological
effects of MF. On the other hand, the fact that modern man is exposed to increasing
number of MF sources due to the use of new technologies in everyday life, industry,
medicine, as well as therapeutic application of MF, makes this topic even more important,
in terms of human health protection.
It is assumed that the interaction between external MF and organisms takes place
at all levels of organization, including the nervous system. Thus, the aim of this study was
to investigate the influence of electromagnetic field (EMF) of different characteristics
(exposure duration, waveform) on spontaneous bioelectrical activity of antennal lobe
neurons of adult Morimus funereus. Neurons activity (background/neuronal population
and those nearest to the recording electrode) was registered through several phases of
exposure of adult longhorn beetles to sine wave or square wave MF of different exposure
duration: 5, 10 and 15 minutes (MP-5, MP-10 and MP-15, respectively). Estimation of MF
influence was based on registered changes of neuronal activity. (I)reversibility of such
changes was tested through comparison the triplets formed by particular experimental
phases. Changes in triplets that follow the time course of the experiment, with reference
to MF influence of different exposure duration (MP-5, MP-10 and MP-15, respectively),
were also tested.
Results of this study showed that the applied MF, regardless of exposure duration
and waveform, modifies the activity of antennal lobe neurons. In sine wave MF, the most
prominent changes are recorded after MP-15, while in square wave MF such changes
were recorded after MP-5. Control activity of the neuronal population was significantly
changed both after MP-5 and MP-10, as well as significantly decreased after MP-15. Sine
wave MF, regardless of exposure duration, significantly inhibits control activity of
nearest neurons. Changes in control neuronal activity are significantly lower in square
wave MF of different exposure duration (MP-5, MP-10, MP-15). Compared to the activity
detected immediately after exposure to sine wave MF, the activity of neuronal population
and nearest neurons is significantly increased 35 minutes after cessation of MF-5 and MP-
10 exposure, respectively. In case of square wave MF, changes in the activity of neuronal
population and nearest neurons are significant 35 minutes after cessation of MF-5
exposure compared to the activity detected immediately after exposure to this MF. On
the other hand, the neuronal activity detected 35 minutes after cessation of MF-10
exposure was significantly higher in the neuronal population and significantly lower in
the nearest neurons compared to the activity detected immediately after exposure to this
square wave MF. Changes in the activity of neurons exposed to sine wave or square wave
MF of different duration were mostly irreversible. Sine wave MF of different duration
does not change reversibility factors between the triplets formed by particular
experimental phases. In contrast, in square wave MF, reversibility factors of the nearest
neurons are significantly changed between the triplets formed by MP-10 and MP-15
experimental phase.
Results of our experiment imply that M. funereus individuals are sensitive to both
sine wave and square wave EMF (2 mT, 50 Hz) of different duration, whereby their
reactions depend on the characteristics of the applied MF and specificity of each
individual. In addition, it can be presumed that adult M. funereus has the ability to detect
and respond to external MF.
Keywords:
Morimus funereus; Electromagnetic field; Spontaneus bioelectrical activity; Extracellular registration; Excitation/inhibition; Irreversibility/reversibilitySource:
University of Belgrade, Faculty of Biology, 2013, 1-160Funding / projects:
- Uticaj magnetnih polja kao ekofiziološkog faktora na različite biološke sisteme i moguća primena u biomedicini (RS-143027)
- The effects of magnetic fields and other environmental stressors on the physiological responses and behavior of different species (RS-MESTD-Basic Research (BR or ON)-173027)
URI
http://eteze.bg.ac.rs/application/showtheses?thesesId=1102https://fedorabg.bg.ac.rs/fedora/get/o:7841/bdef:Content/download
http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1024654002
http://nardus.mpn.gov.rs/123456789/2154
https://radar.ibiss.bg.ac.rs/handle/123456789/2422