Daidzein effects on ACTH cells: Immunohistomorphometric and hormonal study in an animal model of the andropause
2011
Аутори:
Ajdžanović, VladimirŠošić-Jurjević, Branka
Filipović, Branko
Trifunović, Svetlana
Milošević, Verica
Тип документа:
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт:
Daidzein is a potential natural alternative to estradiol during therapy of some malignancies in men. Besides weak inhibition of tyrosine kinase activity, daidzein has a sizeable inhibitory effect on calcium channels. The aim of this study was to examine the effects of daidzein on the immunohistomorphometric features of pituitary adrenocorticotropes (ACTH cells) and circulating levels of ACTH and corticosterone, in comparison with estradiol, in an animal model of the andropause. Sixteen-month-old Wistar rats were divided into sham operated (SO), orchidectomized (Orx), estradiol treated orchidectomized (Orx+E) and daidzein treated orchidectomized (Orx+D) groups. Estradiol (0.625 mg/kg/day) and daidzein (30 mg/kg/day) were administered subcutaneously for three weeks, while the SO and Orx groups received the vehicle alone. ACTH cells were identified by the peroxidase-antiperoxidase (PAP) immunohistochemical procedure. Peripheral circulating concentrations of ACTH and corticosterone were measured by immunoassay. Orchidectomy reduced (p<0.05) the cell volume and volume density of adrenocorticotropes by 11% and 16%, respectively, in comparison to SO rats. In Orx+E rats, the volume density of ACTH cells decreased (p<0.05) by 25%, but the circulating level of ACTH increased (p<0.05) by 29%, compared to Orx rats. Daidzein treatment significantly decreased (p<0.05): volume density of ACTH cells, circulating ACTH and corticosterone by 24%, 48% and 33%, respectively, compared to the Orx group. In conclusion, this study revealed that daidzein negatively modulated the immunohistomorphometric features of ACTH cells and, unlike estradiol, decreased ACTH and corticosterone secretion, in an animal model of the andropause.
Кључне речи:
ACTH; Corticosterone; Daidzein; Estradiol; StressИзвор:
Histology and Histopathology, 2011, 26, 10, 1257-1264Финансирање / пројекти:
- Одговор неуроендокриног система пацова на одабране биљне екстракте, фитоестрогене, стероидне и пептидне хормоне (RS-MESTD-Basic Research (BR or ON)-173009)
DOI: 10.14670/HH-26.1257
ISSN: 0213-3911
PubMed: 21870329
WoS: 000294285900003
Scopus: 2-s2.0-80053333131
URI
http://www.scopus.com/inward/record.url?eid=2-s2.0-80053333131&partnerID=tZOtx3y1https://radar.ibiss.bg.ac.rs/handle/123456789/2463
http://www.hh.um.es/Abstracts/Vol_26/26_10/26_10_1257.htm