Evaluation of functionalized mesoporous silica SBA-15 as a carrier system for Ph 3 Sn(CH 2) 3 OH against the A2780 ovarian carcinoma cell line
2016
Аутори:
Bensing, ChristianMojić, Marija
Gómez-Ruiz, Santiago
Carralero, Sandra
Dojčinović, Biljana
Maksimović-Ivanić, Danijela
Mijatović, Sanja
Kaluđerović, Goran N.
Тип документа:
Чланак у часопису (Објављена верзија)
,
© 2016 The Royal Society of Chemistry
Метаподаци
Приказ свих података о документуАпстракт:
SBA-15|Sn3, a mesoporous silica-based material (derivative of SBA-15) loaded with an organotin compound Ph3Sn(CH2)3OH (Sn3), possesses improved antitumor potential against the A2780 high-grade serous ovarian carcinoma cell line in comparison to Sn3. It is demonstrated that both the compound and the nanostructured material are internalized by the A2780 cells. A similar mode of action of Sn3 and SBA-15|Sn3 against the A2780 cell line was found. Explicitly, induction of apoptosis, caspase 2, 3, 8 and 9 activation, accumulation of cells in the hypodiploid phase as well as accumulation of ROS were observed. Interestingly, Sn3 loaded in the mesoporous silica-based material needed to reach a concentration 3.5 times lower than the IC50 value of the Sn3 compound, pointing out a higher effect of the SBA-15|Sn3 than Sn3 alone. Clonogenic potential, growth in 3D culture as well as mobility of cells were disturbed in the presence of SBA-15|Sn3. Such behavior could be associated with the suppression of p-38 MAPK. Less profound effect of Sn3 compared to SBA-15|Sn3 could be attributed to a different regulation of p-38 and STAT-3, which are mainly responsible for an appropriate cellular response to diverse stimuli or metastatic properties.
Кључне речи:
Cell culture; Cell death; Cells; Cytology; Organometallics; Silica; Tin; Carrier systems; Cellular response; Clonogenic potential; Functionalized; Mesoporous Silica; Mesoporous silica-based materials; Organotin compound; Ovarian carcinoma cellИзвор:
Dalton Transactions, 2016, 45, 47, 18984-18993Финансирање / пројекти:
- Молекуларни механизми физиолошке и фармаколошке контроле инфламације и канцера (RS-MESTD-Basic Research (BR or ON)-173013)
- Ministerio de Economía y Competitividad, Spain (Grant No. CTQ2015-66164-R
- Universidad Rey Juan Carlos-Banco de Santander (Excellence Group QUINANOAP)
DOI: 10.1039/C6DT03519A
ISSN: 1477-9226
PubMed: 27847952
WoS: 000390082900025
Scopus: 2-s2.0-85000774313
URI
http://xlink.rsc.org/?DOI=C6DT03519Ahttps://www.scopus.com/record/display.uri?eid=2-s2.0-85000774313&origin=SingleRecordEmailAlert&dgcid=scalert_sc_search_email&txGid=691E27A4B52E4CB98111082A19AFDEEC.wsnAw8kcdt7IPYLO0V48gA%3A11#
https://radar.ibiss.bg.ac.rs/handle/123456789/2481