CXCL12 protects pancreatic β-cells from oxidative stress by a Nrf2-induced increase in catalase expression and activity
2016
Аутори:
Dinić, SvetlanaGrdović, Nevena
Uskoković, Aleksandra
Đorđević, Miloš
Mihailović, Mirjana
Arambašić Jovanović, Jelena
Poznanović, Goran
Vidaković, Melita
Тип документа:
Чланак у часопису (Објављена верзија)
,
©2016 The Japan Academy
Метаподаци
Приказ свих података о документуАпстракт:
Due to intrinsically low levels of antioxidant enzyme expression and activity, insulin producing pancreatic β-cells are particularly susceptible to free radical attack. In diabetes mellitus, which is accompanied by high levels of oxidative stress, this feature of β-cells significantly contributes to their damage and dysfunction. In light of the documented pro-survival effect of chemokine C-X-C Ligand 12 (CXCL12) on pancreatic β-cells, we examined its potential role in antioxidant protection. We report that CXCL12 overexpression enhanced the resistance of rat insulinoma (Rin-5F) and primary pancreatic islet cells to hydrogen peroxide (H2O2). CXCL12 lowered the levels of DNA damage and lipid peroxidation and preserved insulin expression. This effect was mediated through an increase in catalase (CAT) activity. By activating downstream p38, Akt and ERK kinases, CXCL12 facilitated Nrf2 nuclear translocation and enhanced its binding to the CAT gene promoter, inducing constitutive CAT expression and activity that was essential for protecting β-cells from H2O2.
Кључне речи:
CXCL12; catalase; pancreatic β-cells; Nrf2; H2O2 treatmentФинансирање / пројекти:
- Сигнални молекули у дијабетесу: идентификација потенцијалних биолошких маркера укључених у модификацију и интеграцију сигналних путева у циљу предикције и интервенције у дијабетесу (RS-MESTD-Basic Research (BR or ON)-173020)
У:
- Proceedings of the Japan Academy, Series B (2016), 92(9): 436-454
DOI: 10.2183/pjab.92.436
ISSN: 0386-2208
PubMed: 27840391
WoS: 000391163600004
Scopus: 2-s2.0-85002962802
URI
https://www.jstage.jst.go.jp/article/pjab/92/9/92_PJA9209B-05/_articlehttps://www.scopus.com/record/display.uri?eid=2-s2.0-85002962802&origin=SingleRecordEmailAlert&dgcid=scalert_sc_search_email&txGid=FBB4104D27B7BC3C905C7DEC47444824.wsnAw8kcdt7IPYLO0V48gA%3A98
https://radar.ibiss.bg.ac.rs/handle/123456789/2491