Evaluation of genotoxic potential throughout the upper and middle stretches of Adige river basin
2016
Аутори:
Kračun-Kolarević, MargaretaKolarević, Stoimir
Jovanović, Jovana
Marković, Vanja
Ilić, Marija
Simonović, Predrag
Simić, Vladica
Gačić, Zoran
Diamantini, Elena
Stella, Elisa
Petrović, Mira
Majone, Bruno
Bellin, Alberto
Paunović, Momir
Vuković-Gačić, Branka
Тип документа:
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт:
In this study a comprehensive genotoxicological survey throughout the upper and middle stretches of Adige river basin is presented. The study was carried out at 7 sites located along the Adige main course and one the most significant tributaries, the Noce creek, both presenting different levels of pollution pressure. To give an insight into the nature of the genotoxic activity we employed the battery of prokaryotic and eukaryotic assays. Mutagenicity in water samples was evaluated by SOS/umuC test in Salmonella typhimurium TA1535/pSK1002. The level of DNA damage as a biomarker of exposure (comet assay) and biomarker of effect (micronucleus assay) and the level of oxidative stress as well (Fpg - modified comet assay) were studied in blood cells of Salmo cenerinus Nardo, 1847 and Salmo marmoratus Cuvier, 1829. Within the applied bioassays, comet assay showed the highest potential for discriminating the sampling sites which are under lesser extent of pressure (sampling sites 1-Barnes at Bresimo and 4-Noce downstream S. Giustina) from the sites under high pressure (sampling sites 5-Noce at Mezzolombardo and 6/7-Adige upstream and downstream municipality of Trento). Significant correlation between the standard and Fpg - modified comet assay indicated that oxidative stress could be a major contributor to observed DNA damage in collected specimens.
Кључне речи:
Trout; Adige River; Ecogenotoxicology; Comet assay; MicronucleusФинансирање / пројекти:
- Managing the effects of multiple stressors on aquatic ecosystems under water scarcity (EU-FP7-603629)
- This study represents a part of activities within the Projects funded by the Ministry of Education, Science and Technological Development of the Republic of Serbia and bilateral project Serbia and Slovenia BI-RS/14-15-013
У:
- Science of The Total Environment (2016), 571: 1383-1391
DOI: 10.1016/j.scitotenv.2016.07.099
ISSN: 0048-9697
PubMed: 27450952
WoS: 000383930400138
Scopus: 2-s2.0-84979519348
URI
http://linkinghub.elsevier.com/retrieve/pii/S0048969716315455https://radar.ibiss.bg.ac.rs/handle/123456789/2505