Comparative studies on osmosis based encapsulation of sodium diclofenac in porcine and outdated human erythrocyte ghosts
2016
Аутори:
Bukara, KatarinaDrvenica, Ivana
Ilić, Vesna
Stančić, Ana
Mišić, Danijela
Vasić, Borislav
Gajić, Radoš
Vučetić, Dušan
Kiekens, Filip
Bugarski, Branko
Тип документа:
Чланак у часопису (Објављена верзија)
,
© 2016 Elsevier B.V.
Метаподаци
Приказ свих података о документуАпстракт:
The objective of our study was to develop controlled drug delivery system based on erythrocyte ghosts for amphiphilic compound sodium diclofenac considering the differences between erythrocytes derived from two readily available materials – porcine slaughterhouse and outdated transfusion human blood. Starting erythrocytes, empty erythrocyte ghosts and diclofenac loaded ghosts were compared in terms of the encapsulation efficiency, drug releasing profiles, size distribution, surface charge, conductivity, surface roughness and morphology. The encapsulation of sodium diclofenac was performed by an osmosis based process – gradual hemolysis. During this process sodium diclofenac exerted mild and delayed antihemolytic effect and increased potassium efflux in porcine but not in outdated human erythrocytes. FTIR spectra revealed lack of any membrane lipid disorder and chemical reaction with sodium diclofenac in encapsulated ghosts. Outdated human erythrocyte ghosts with detected nanoscale damages and reduced ability to shrink had encapsulation efficiency of only 8%. On the other hand, porcine erythrocyte ghosts had encapsulation efficiency of 37% and relatively slow drug release rate. More preserved structure and functional properties of porcine erythrocytes related to their superior encapsulation and release performances, define them as more appropriate for the usage in sodium diclofenac encapsulation process.
Кључне речи:
Controlled drug delivery; Encapsulation; Erythrocyte membranes; Gradual hypotonic hemolysis; Pharmaceutical biotechnology; Slaughterhouse bloodИзвор:
Journal of Biotechnology, 2016, 240, 14-22Финансирање / пројекти:
- Развој нових инкапсулационих и ензимских технологија за производњу биокатализатора и биолошки активних компонената хране у циљу повећања њене конкурентности, квалитета и безбедности (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46010)
- Физика уређених наноструктура и нових материјала у фотоници (RS-MESTD-Basic Research (BR or ON)-171005)
DOI: 10.1016/j.jbiotec.2016.10.017
ISSN: 0168-1656
WoS: 000389116700002
Scopus: 2-s2.0-84992192143
URI
http://linkinghub.elsevier.com/retrieve/pii/S0168165616315735https://www.scopus.com/record/display.uri?eid=2-s2.0-84992192143&origin=SingleRecordEmailAlert&txGid=694651161B2E63C5600F6DE538B0ACEC.wsnAw8kcdt7IPYLO0V48gA:3
https://radar.ibiss.bg.ac.rs/handle/123456789/2525