Comparative studies on osmosis based encapsulation of sodium diclofenac in porcine and outdated human erythrocyte ghosts
2016
Authors:
Bukara, KatarinaDrvenica, Ivana
Ilić, Vesna
Stančić, Ana
Mišić, Danijela
Vasić, Borislav
Gajić, Radoš
Vučetić, Dušan
Kiekens, Filip
Bugarski, Branko
Document Type:
Article (Published version)
,
© 2016 Elsevier B.V.
Metadata
Show full item recordAbstract:
The objective of our study was to develop controlled drug delivery system based on erythrocyte ghosts for amphiphilic compound sodium diclofenac considering the differences between erythrocytes derived from two readily available materials – porcine slaughterhouse and outdated transfusion human blood. Starting erythrocytes, empty erythrocyte ghosts and diclofenac loaded ghosts were compared in terms of the encapsulation efficiency, drug releasing profiles, size distribution, surface charge, conductivity, surface roughness and morphology. The encapsulation of sodium diclofenac was performed by an osmosis based process – gradual hemolysis. During this process sodium diclofenac exerted mild and delayed antihemolytic effect and increased potassium efflux in porcine but not in outdated human erythrocytes. FTIR spectra revealed lack of any membrane lipid disorder and chemical reaction with sodium diclofenac in encapsulated ghosts. Outdated human erythrocyte ghosts with detected nanoscale damages and reduced ability to shrink had encapsulation efficiency of only 8%. On the other hand, porcine erythrocyte ghosts had encapsulation efficiency of 37% and relatively slow drug release rate. More preserved structure and functional properties of porcine erythrocytes related to their superior encapsulation and release performances, define them as more appropriate for the usage in sodium diclofenac encapsulation process.
Keywords:
Controlled drug delivery; Encapsulation; Erythrocyte membranes; Gradual hypotonic hemolysis; Pharmaceutical biotechnology; Slaughterhouse bloodSource:
Journal of Biotechnology, 2016, 240, 14-22Funding / projects:
- Novel encapsulation and enzyme technologies for designing of new biocatalysts and biologically active compounds targeting enhancement of food quality, safety and competitiveness (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46010)
- Physics of Ordered Nanostructures and New Materials in Photonics (RS-MESTD-Basic Research (BR or ON)-171005)
DOI: 10.1016/j.jbiotec.2016.10.017
ISSN: 0168-1656
WoS: 000389116700002
Scopus: 2-s2.0-84992192143
URI
http://linkinghub.elsevier.com/retrieve/pii/S0168165616315735https://www.scopus.com/record/display.uri?eid=2-s2.0-84992192143&origin=SingleRecordEmailAlert&txGid=694651161B2E63C5600F6DE538B0ACEC.wsnAw8kcdt7IPYLO0V48gA:3
https://radar.ibiss.bg.ac.rs/handle/123456789/2525