Association of CXCL12 gene promoter methylation with periodontitis in patients with diabetes mellitus type 2
2016
Аутори:
Grdović, NevenaRajić, Jovana
Matić Petrović, Sanja
Dinić, Svetlana
Uskoković, Aleksandra
Mihailović, Mirjana
Arambašić Jovanović, Jelena
Tolić, Anja
Pucar, Ana
Milašin, Jelena
Vidaković, Melita
Тип документа:
Чланак у часопису (Објављена верзија)
,
© 2016 Elsevier Ltd.
Метаподаци
Приказ свих података о документуАпстракт:
Objectives CXCL12 is widely expressed, constitutive chemokine involved in tissue repair and regeneration, while the extent of its expression is important in various chronic inflammatory conditions. Involvement of DNA methylation in CXCL12 gene suppression (CXCL12) has been shown in malignancy and some autoimmune diseases. The aim of this study was to investigate whether the alterations in DNA methylation of CXCL12 are also involved in progression of periodontitis in combination with diabetes, as these chronic inflammatory conditions are strongly interrelated. Design Study included 72 subjects divided in three groups: healthy control (C, n = 21), periodontitis (P, n = 29) and diabetes/periodontitis group (D/P, n = 22). DNA extracted from epithelial cells obtained by sterile cotton swabs from buccal mucosa was subjected to methylation specific polymerase chain reaction (MSP) to obtain DNA methylation pattern of CXCL12 promoter. Results CXCL12 promoter was predominantly unmethylated in all groups. However, increase in the frequency of the methylated form and increase in percent of methylation of CXCL12 promoter in periodontitis and diabetes/periodontitis group compared to control group were found, although without statistical significance. However, statistically significant increase in Tm of MSP products in diabetes/periodontitis group was observed. Correlation analysis revealed statistically significant relationship between the extent of DNA methylation of the CXCL12 promoter and periodontal parameters, as well as between DNA methylation of CXCL12 and glycosylated hemoglobin. Conclusion Presented results suggest that chronic inflammation contributes to the change of CXCL12 DNA methylation in buccal cells and that DNA methylation profile of CXCL12 promoter plays important role in development and progression of periodontal disease.
Кључне речи:
CXCL12; DNA methylation; Periodontitis; Diabetes; Buccal cellsИзвор:
Archives of Oral Biology, 2016, 72, 124-133Финансирање / пројекти:
- Сигнални молекули у дијабетесу: идентификација потенцијалних биолошких маркера укључених у модификацију и интеграцију сигналних путева у циљу предикције и интервенције у дијабетесу (RS-MESTD-Basic Research (BR or ON)-173020)
DOI: 10.1016/j.archoralbio.2016.08.025
ISSN: 0003-9969
PubMed: 27580404
WoS: 000387835400017
Scopus: 2-s2.0-84983475101
URI
http://linkinghub.elsevier.com/retrieve/pii/S0003996916302229https://www.scopus.com/record/display.uri?eid=2-s2.0-84983475101&origin=resultslist&sort=plf-f&src=s&st1=Association+of+CXCL12+gene+promoter+methylation+with+periodontitis+in+patients+with+diabetes+mellitus&st2=&sid=55EA0C8D3BB1AC9032AD3C39F3D96943.wsnAw8kcdt7IPYLO0V48gA%3A420&sot=b&sdt=b&sl=116&s=TITLE-ABS-KEY%28Association+of+CXCL12+gene+promoter+methylation+with+periodontitis+in+patients+with+diabetes+mellitus%29&relpos=0&citeCnt=0&searchTerm=
https://radar.ibiss.bg.ac.rs/handle/123456789/2543