Two opposite dose-dependent effects of diazinon on the motor activity of the rat ileum
2017
Authors:
Trailović, Saša M.Marjanović, Đorđe S.
Vidonja Uzelac, Teodora
Milovanović, Mirjana
Nedeljković Trailović, Jelena
Document Type:
Article (Published version)
,
© 2017 Elsevier Ltd.
Metadata
Show full item recordAbstract:
Acute poisoning with OPs may lead to a range of neurological effects, which cannot be explained by AChE inhibition alone. Several OPs interact directly with cholinergic receptors in mammals, but such data does not exist for invertebrates. The aim of current study was to investigate the direct and indirect effects of diazinon on the contractions of rat ileum and to compare those effects on the nervemuscle preparation of the Ascaris suum. In the presence of increasing concentrations of diazinon (3, 10 and 30 nM), EFS-induced ileal contractions were increased significantly. In the same preparation, diazinon 3 nM, significantly increased contractions induced by EFS, but did not affect the contractions caused by 5MFI. Contrarily, 1 μM of diazinon significantly and reversibly inhibited the EFS-induced ileal contractions. Diazinon exhibited competitive and non-competitive inhibitions of 5MFI induced contractions. The control EC50 of 5MFI was 2.48 μM with Rmax = 1.88 g. In the presence of diazinon, EC50 was 12.45 μM, while Rmax was reduced to 0.43 g. After washing, the EC50 and Rmax values were again closer to the control level (3.80 μM and 1.04 g). Diazinon 1 μM did not inhibit Ascaris suum contractions caused by ACh, but it increased the Rmax. Diazinon in our study exhibits two opposite effects on the motor activity of the ileum. In low nanomolar concentrations the dominat is its effect on AChE and the stimulation of contractions. Furthermore, in concentrations that approach micromolar values diazinon has a direct inhibitory effect on muscarinic receptors. The direct inhibitory effect of diazinon on A. suum contractions was not found.
Keywords:
Ascaris suum; Diazinon; Ileum; Muscarinic acetylcholine receptorFunding / projects:
- Development of herbal medicines and biocides on the basis of Carvacrol, Thymol and Cinnamaldehyde for use in veterinary medicine, animal husbandry and food production without harmful residues (RS-MESTD-Technological Development (TD or TR)-31087)
In:
- Research in Veterinary Science (2017), 112(2): 18-25
DOI: 10.1016/j.rvsc.2017.01.002
ISSN: 0034-5288
PubMed: 28107667
WoS: 000403860900004
Scopus: 2-s2.0-85009518367
URI
http://linkinghub.elsevier.com/retrieve/pii/S0034528817300103https://radar.ibiss.bg.ac.rs/handle/123456789/2552