Extracellular ATP induces graded reactive response of astrocytes and strengthens their antioxidative defense in vitro
2017
Autori:
Adžić, MarijaStevanović, Ivana
Josipović, Nataša
Laketa, Danijela
Lavrnja, Irena
Bjelobaba, Ivana
Božić, Iva
Jovanović, Marija
Milošević, Milena
Nedeljković, Nadežda
Tip dokumenta:
Članak u časopisu (Objavljena verzija)
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© 2016 Wiley Periodicals, Inc.
Metapodaci
Prikaz svih podataka o dokumentuApstrakt:
It is widely accepted that adenosine triphosphate (ATP) acts as a universal danger-associated molecular pattern with several known mechanisms for immune cell activation. In the central nervous system, ATP activates microglia and astrocytes and induces a neuroinflammatory response. The aim of the present study was to describe responses of isolated astrocytes to increasing concentrations of ATP (5 µM to 1 mM), which were intended to mimic graded intensity of the extracellular stimulus. The results show that ATP induces graded activation response of astrocytes in terms of the cell proliferation, stellation, shape remodeling, and underlying actin and GFAP filament rearrangement, although the changes occurred without an apparent increase in GFAP and actin protein expression. On the other hand, ATP in the range of applied concentrations did not evoke IL-1β release from cultured astrocytes, nor did it modify the release from LPS and LPS+IFN-γ–primed astrocytes. ATP did not promote astrocyte migration in the wound-healing assay, nor did it increase production of reactive oxygen and nitrogen species and lipid peroxidation. Instead, ATP strengthened the antioxidative defense of astrocytes by inducing Cu/ZnSOD and MnSOD activities and by increasing their glutathione content. Our current results suggest that although ATP triggers several attributes of activated astrocytic phenotype with a magnitude that increases with the concentration, it is not sufficient to induce full-blown reactive phenotype of astrocytes in vitro. © 2016 Wiley Periodicals, Inc.
Ključne reči:
Antioxidative defense; ATP; IL-1β; Reactive astrocytes; Reactive gliosisIzvor:
Journal of Neuroscience Research, 2017, 95, 4, 1053-1066Finansiranje / projekti:
- Ćelijska i molekulska osnova neuroinflamacije: potencijala ciljna mesta za translacionu medicinu i terapiju (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41014)
DOI: 10.1002/jnr.23950
ISSN: 0360-4012
PubMed: 27714837