Cytotoxic activity of Nepeta rtanjensis Diklić & Milojević essential oil and its mode of action
2017
Authors:
Skorić, MarijanaGligorijević, Nevenka
Čavić, Milena
Todorović, Slađana
Janković, Radmila
Ristić, Mihailo
Mišić, Danijela
Radulović, Siniša
Document Type:
Article (Published version)
,
© 2017 Elsevier B.V.
Metadata
Show full item recordAbstract:
Nepeta rtanjensis Diklić & Milojević (fam. Lamiaceae) is an endemic, critically endangered plant, protected by the law in Serbia. Various biological activities have been ascribed to its major constituents, nepetalactones. In this study we describe for the first time cytotoxic activity of N. rtanjensis essential oil (EO), obtained from field cultivated plants, which was found to be especially rich in trans,cis-nepetalactone. MTT assays indicated that after 72 h of treatment the EO exhibited cytotoxic activity on investigated cancer cell lines: HeLa, K562, A549, LS-174 and MDA-MB-231. Normal cell line (MRC-5) was the least sensitive to the treatment and IC50 value for this cell line was not reached within the tested range of EO concentrations (up to 0.1 μL/mL). Analysis of morphological changes of treated cells confirmed the higher sensitivity of tumor cells than normal cells to the tested EO. Application of N. rtanjensis EO resulted in the appearance of morphological changes in tested cancer cell lines characteristic for apoptotic cell death, and induced perturbations of the cell cycle of HeLa cells. In addition, upregulation of Bax and p53, and downregulation of Bcl-2, and Skp2 genes, involved in apoptotic signalling cascades, confirmed an apoptosis-inducing effect of N. rtanjensis EO on HeLa cells. Presented results highlighted the potential of N. rtanjensis EO in anticancer therapy.
Keywords:
Anticancer activity; Apoptosis; Nepeta rtanjensis; trans,cis-nepetalactoneSource:
Industrial Crops and Products, 2017, 100, 163-170Funding / projects:
- Physiological, chemical and molecular analysis of the diversity of selected rare and endangered plant species and application of biotechnology for ex situ conservation and production of biologically active compounds (RS-MESTD-Basic Research (BR or ON)-173024)
- Pharmacodynamic and pharmacogenomic research of new drugs in the treatment of solid tumors (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41026)
- Investigation on the medicinal plants: morphological, chemical and pharmacological characterisation (RS-MESTD-Basic Research (BR or ON)-173021)
DOI: 10.1016/j.indcrop.2017.02.027
ISSN: 0926-6690
WoS: 000397687200018
Scopus: 2-s2.0-85014170661
URI
http://linkinghub.elsevier.com/retrieve/pii/S0926669017301309https://radar.ibiss.bg.ac.rs/handle/123456789/2609