The effects of N-acetylcysteine on cisplatin-induced changes of cardiodynamic parameters within coronary autoregulation range in isolated rat hearts.
2016
Аутори:
Rosić, GvozdenSelaković, Dragica
Joksimović, Jovana
Srejović, Ivan
Živković, Vladimir
Tatalović, Nikola
Oreščanin Dušić, Zorana
Mitrović, Slobodanka
Ilić, Milena
Jakovljević, Vladimir
Тип документа:
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт:
The aim of this study was to evaluate the effects of chronic NAC administration along with cisplatin on cisplatin-induced cardiotoxicity by means of coronary flow (CF), cardiodynamic parameters, oxidative stress markers and morphological changes in isolated rat heart. Isolated hearts of Wistar albino rats (divided into four groups: control, cisplatin, NAC and cisplatin+NAC group) were perfused according to Langendorff technique at constant coronary perfusion pressure starting at 50 and gradually increased to 65, 80, 95 and 110 cm H2O to evaluate cardiodynamic parameters within autoregulation range. Samples of coronary venous effluent (CVE) were collected for determination of CF and biochemical assays, and heart tissue samples for biochemical assays and histopathological examination. Cisplatin treatment decreased CF and heart rate, and increased left ventricular systolic pressure and maximum left ventricular pressure development rate. Cisplatin increased H2O2 and TBARS, but decreased NO2(-) levels in CVE. In tissue samples, cisplatin reduced pathological alterations in myocardium and coronary vessels, with no changes in the amount of total glutathione, as well as in activity of glutathione peroxidase and glutathione reductase. NAC coadministration, by reducing oxidative damage, attenuated cisplatin-induced changes of cardiodynamic and oxidative stress parameters, as well as morphological changes in myocardium and coronary vasculature.
Кључне речи:
Cisplatin; Coronary autoregulation; Histology; Isolated rat heart; N-acetylcysteine; Oxidative stressИзвор:
Toxicology letters, 2016, 242, 34-46Финансирање / пројекти:
- Примена биомедицинског инжењеринга у претклиничкој и клиничкој пракси (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41007)
DOI: 10.1016/j.toxlet.2015.11.028
ISSN: 0378-4274
PubMed: 26656795
WoS: 000367654400004
Scopus: 2-s2.0-84949638402
URI
http://linkinghub.elsevier.com/retrieve/pii/S0378427415301223http://www.ncbi.nlm.nih.gov/pubmed/26656795
https://radar.ibiss.bg.ac.rs/handle/123456789/2612