Production of phenolic compounds, antioxidant and antimicrobial activities in hairy root and shoot cultures of Hypericum perforatum L.
2017
Аутори:
Tusevski, OliverVinterhalter, Branka
Krstić Milošević, Dijana
Soković, Marina
Ćirić, Ana
Vinterhalter, Dragan
Zdravković-Korać, Snežana
Petreska Stanoeva, Jasmina
Stefova, Marina
Gadzovska Simic, Sonja
Тип документа:
Чланак у часопису (Објављена верзија)
,
© 2016, Springer Science+Business Media Dordrecht
Метаподаци
Приказ свих података о документуАпстракт:
Three hairy root clones of Hypericum perforatum (HR 2, HR 15 and HR 27) transformed with Agrobacterium rhizogenes A4M70GUS and their corresponding regenerated shoot culture clones (HRRS) were compared for differences in growth, production of phenolic compounds, antioxidant and antimicrobial activities. Transgenic clones were selected on the basis of morphological evaluation, genetic and molecular analyses. The clone HR 2 had the highest biomass accumulation, while HR 27 showed the highest shoot regeneration potential. The total phenolics and flavan-3-ols were enhanced in all tested transgenic cultures, while total flavonoids and hypericins were augmented in HRRS clones compared to non-transformed shoots. The HRRS clones produced substantial amounts of chlorogenic acid and 3-p-coumaroylquinic acid. Regarding the flavonoids, they produced significant contents of luteolin hexoside (HRRS 2), quercitrin and quercetin (HRRS 15) and isoquercetin (HRRS 27), while HR 2 and 15 accumulated 4-O-methylkaempferol-O-hexoside and quercetin 6-C-glucoside, respectively. The HR 15 was promising for the production of catechin and procyanidin derivatives and together with its HRRS clone exhibited a high potential for hyperforin and adhyperforin production. All identified naphtodianthrones were confirmed in HRRS 2 and 15 clones. Among xanthones, mangiferin was found as the major compound in HRRS, while trihydroxy-1-metoxy-C-prenyl xanthone was dominant in HR clones. Antimicrobial activity of transgenic cultures revealed that HRRS 15 strongly inhibited the growth of Bacillus cereus, Micrococcus flavus, Pseudomonas aeruginosa and Escherichia coli. Altogether, H. perforatum HR and HRRS cultures could be proposed as promising experimental systems for enhanced production of phenolic compounds with antioxidant and antibacterial properties.
Кључне речи:
Antioxidant and antimicrobial activities; Hairy root-regenerated shoots; Hairy roots; Hypericum perforatum; Phenolic compoundsИзвор:
Plant Cell, Tissue and Organ Culture (PCTOC), 2017, 128, 3, 589-605Финансирање / пројекти:
- Биотехнологија ин витро - гајене, лековите и угрожене биљне врсте (RS-173015)
- Карактеризација и примена метаболита гљива и утврђивање потенцијала нових биофунгицида (RS-173032)
DOI: 10.1007/s11240-016-1136-9
ISSN: 0167-6857
WoS: 000395207000007
Scopus: 2-s2.0-84997447762
URI
http://link.springer.com/10.1007/s11240-016-1136-9https://radar.ibiss.bg.ac.rs/handle/123456789/2688