Alpha-1-Antitrypsin Antagonizes Cisplatin-Induced Cytotoxicity in Prostate Cancer (PC3) and Melanoma Cancer (A375) Cell Lines
2017
Authors:
Ljujić, MilaMijatović, Sanja
Bulatović, Mirna
Mojić, Marija
Maksimović-Ivanić, Danijela
Radojković, Dragica
Topić, Aleksandra
Document Type:
Article (Published version)
,
© 2016, Arányi Lajos Foundation
Metadata
Show full item recordAbstract:
Increased circulating alpha-1-antitrypsin (AAT) correlates with cancer stage/aggressiveness, but its role in cancer biology is unclear. We revealed antagonistic effect of AAT to cisplatin-induced cytotoxicity in prostate (PC3) and melanoma (A375) cancer cell lines. Moreover, AAT abrogated cytotoxicity of MEK inhibitor U0126 in PC3 cell line. Weaker antagonistic effect of AAT on cytotoxicity of PI3/Akt and NF-kB inhibitors was also observed. In addition, cisplatin increased AAT gene expression in transfected PC3 cells. However, AAT derived from transfected PC3 cells did not antagonize cisplatin-induced cytotoxicity. In conclusion, these results suggest possible association between high circulating AAT and cisplatin resistance.
Keywords:
Alpha-1-antitrypsin; Cisplatin; Melanoma cancer; Prostate cancerSource:
Pathology & Oncology Research, 2017, 23, 2, 335-343Funding / projects:
- Molecular mechanisms of physiological and pharmacological control of inflammation and cancer (RS-MESTD-Basic Research (BR or ON)-173013)
- Complex diseases as a model system for phenotype modulation- structural and functional analysis of molecular biomarkers (RS-MESTD-Basic Research (BR or ON)-173008)
DOI: 10.1007/s12253-016-0104-3
ISSN: 1219-4956
PubMed: 27617337
WoS: 000399000200014
Scopus: 2-s2.0-85016626907
URI
http://link.springer.com/10.1007/s12253-016-0104-3https://radar.ibiss.bg.ac.rs/handle/123456789/2729