Приказ основних података о документу
Early energy metabolism-related molecular events in skeletal muscle of diabetic rats: The effects of l -arginine and SOD mimic
dc.creator | Stančić, Ana | |
dc.creator | Filipović, Miloš | |
dc.creator | Ivanović-Burmazović, Ivana | |
dc.creator | Mašović, Sava | |
dc.creator | Janković, Aleksandra | |
dc.creator | Otašević, Vesna | |
dc.creator | Korać, Aleksandra | |
dc.creator | Buzadžić, Biljana | |
dc.creator | Korać, Bato | |
dc.date.accessioned | 2017-11-23T11:28:48Z | |
dc.date.available | 2900-01-01 | |
dc.date.issued | 2017 | |
dc.identifier.issn | 0009-2797 | |
dc.identifier.uri | http://linkinghub.elsevier.com/retrieve/pii/S0009279717300030 | |
dc.identifier.uri | https://radar.ibiss.bg.ac.rs/handle/123456789/2766 | |
dc.description.abstract | Considering the vital role of skeletal muscle in control of whole-body metabolism and the severity of long-term diabetic complications, we aimed to reveal the molecular pattern of early diabetes-related skeletal muscle phenotype in terms of energy metabolism, focusing on regulatory mechanisms, and the possibility to improve it using two redox modulators, L-arginine and superoxide dismutase (SOD) mimic. Alloxan-induced diabetic rats (120 mg/kg) were treated with L-arginine or the highly specific SOD mimic, M40403, for 7 days. As appropriate controls, non-diabetic rats received the same treatments. We found that L-arginine and M40403 restored diabetes-induced impairment of phospho-5′-AMP-activated protein kinase α (AMPKα) signaling by upregulating AMPKα protein itself and its downstream effectors, peroxisome proliferator-activated receptor-γ coactivator-1α and nuclear respiratory factor 1. Also, there was a restitution of the protein levels of oxidative phosphorylation components (complex I, complex II and complex IV) and mitofusin 2. Furthermore, L-arginine and M40403 induced translocation of glucose transporter 4 to the membrane and upregulation of protein of phosphofructokinase and acyl coenzyme A dehydrogenase, diminishing negative diabetic effects on limiting factors of glucose and lipid metabolism. Both treatments abolished diabetes-induced downregulation of sarcoplasmic reticulum calcium-ATPase proteins (SERCA 1 and 2). Similar effects of L-arginine and SOD mimic treatments suggest that disturbances in the superoxide/nitric oxide ratio may be responsible for skeletal muscle mitochondrial and metabolic impairment in early diabetes. Our results provide evidence that L-arginine and SOD mimics have potential in preventing and treating metabolic disturbances accompanying this widespread metabolic disease. | en |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173054/RS// | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173055/RS// | |
dc.relation | European Cooperation in Science and Research (COST Action BM1203) | |
dc.rights | restrictedAccess | |
dc.source | Chemico-Biological Interactions | |
dc.subject | Diabetes | |
dc.subject | Skeletal muscle | |
dc.subject | Redox regulation | |
dc.subject | L-Arginine | |
dc.subject | SOD mimic | |
dc.title | Early energy metabolism-related molecular events in skeletal muscle of diabetic rats: The effects of l -arginine and SOD mimic | en |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Оташевић, Весна; Кораћ, Бато; Бузаджић, Биљана; Кораћ, Aлександра; Јанковић, Aлександра; Машовић, Сава; Ивановић-Бурмазовић, Ивана; Филиповић, Милош; Станчић, Aна; | |
dc.rights.holder | © 2017 Elsevier B.V. | |
dc.citation.volume | 272 | |
dc.description.other | Chemico-Biological Interactions (2017), 272: 188-196 | |
dc.identifier.doi | 10.1016/j.cbi.2017.05.003 | |
dc.identifier.pmid | 28483572 | |
dc.identifier.scopus | 2-s2.0-85019714424 | |
dc.identifier.wos | 000404198300021 | |
dc.citation.apa | Stancic, A., Filipovic, M., Ivanovic-Burmazovic, I., Masovic, S., Jankovic, A., Otasevic, V., Korac, A., et al. (2017). Early energy metabolism-related molecular events in skeletal muscle of diabetic rats: The effects of l -arginine and SOD mimic. Chemico-Biological Interactions, 272, 188–196. | |
dc.citation.vancouver | Stancic A, Filipovic M, Ivanovic-Burmazovic I, Masovic S, Jankovic A, Otasevic V, Korac A, Buzadzic B, Korac B. Early energy metabolism-related molecular events in skeletal muscle of diabetic rats: The effects of l -arginine and SOD mimic. Chem Biol Interact. 2017;272:188–96. | |
dc.citation.spage | 188 | |
dc.citation.epage | 196 | |
dc.type.version | publishedVersion | en |
dc.citation.rank | M21 |