Effect of elicitors on xanthone accumulation and biomass production in hairy root cultures of Gentiana dinarica
2017
Autori:
Krstić Milošević, DijanaJanković, Teodora
Uzelac, Branka
Vinterhalter, Dragan
Vinterhalter, Branka
Tip dokumenta:
Članak u časopisu (Objavljena verzija)
,
© Springer Science+Business Media B.V. 2017
Metapodaci
Prikaz svih podataka o dokumentuApstrakt:
The effect of abiotic (salicylic acid, jasmonic acid and methyl jasmonate) and biotic (chitosan and yeast extract) elicitors on the growth and xanthone accumulation in two hairy root clones of Gentiana dinarica Beck. was studied. The obtained results showed that clone 3 was more responsive to elicitor treatment than clone D. The production of dominant xanthone norswertianin-1-O-primeveroside was not significantly affected by either of the abiotic elicitor tested but was stimulated with chitosan treatment. The highest concentrations of all elicitors strongly increased the content of xanthone aglycone norswertianin, but simultaneously reducing the production of its glycoside norswertianin-1-O-primeveroside. The most efficient in enhancing norswertianin production was a 7-day treatment with salicylic acid (200 μM) and chitosan (50 mg l− 1), which yielded a 7.7- and a 24-fold increase in norswertianin content, respectively. In addition, treatment with biotic elicitors caused the occurrence of new xanthone compounds that were not detected in other samples. Free radical scavenging activity of xanthones was carried out by DPPH assay, and norswertianin showed the strongest activity.
Ključne reči:
Abiotic and biotic elicitation; DPPH radical scavenging; Gentiana dinarica; NorswertianinIzvor:
Plant Cell, Tissue and Organ Culture (PCTOC), 2017, 130, 631-640Finansiranje / projekti:
- Biotehnologija in vitro - gajene, lekovite i ugrožene biljne vrste (RS-MESTD-Basic Research (BR or ON)-173015)
DOI: 10.1007/s11240-017-1252-1
ISSN: 0167-6857
WoS: 000408008200015
Scopus: 2-s2.0-85021089437
URI
https://link.springer.com/article/10.1007/s11240-017-1252-1https://radar.ibiss.bg.ac.rs/handle/123456789/2773