Short-term fasting promotes insulin expression in rat hypothalamus
2017
Аутори:
Dakić, Tamara B.Jevđović, Tanja V.
Perić, Mina I.
Bjelobaba, Ivana
Markelić, Milica B.
Milutinović, Bojana S.
Lakić, Iva V.
Jasnić, Nebojša I.
Đorđević, Jelena D.
Vujović, Predrag Z.
Тип документа:
Чланак у часопису (Објављена верзија)
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© 2017 Federation of European Neuroscience Societies and John Wiley & Sons Ltd
Метаподаци
Приказ свих података о документуАпстракт:
In the hypothalamus, insulin takes on many roles involved in energy homoeostasis. Therefore, the aim of this study was to examine hypothalamic insulin expression during the initial phase of the metabolic response to fasting. Hypothalamic insulin content was assessed by both radioimmunoassay and Western blot. The relative expression of insulin mRNA was examined by qPCR. Immunofluorescence and immunohistochemistry were used to determine the distribution of insulin immunopositivity in the hypothalamus. After 6-h fasting, both glucose and insulin levels were decreased in serum but not in the cerebrospinal fluid. Our study showed for the first time that, while the concentration of circulating glucose and insulin decreased, both insulin mRNA expression and insulin content in the hypothalamic parenchyma were increased after short-term fasting. Increased insulin immunopositivity was detected specifically in the neurons of the hypothalamic periventricular nucleus and in the ependymal cells of fasting animals. These novel findings point to the complexity of mechanisms regulating insulin expression in the CNS in general and in the hypothalamus in particular.
Кључне речи:
Fasting; Glucose; Hypothalamus; Insulin; RatФинансирање / пројекти:
- Ефекат метаболичких и неметаболичких стресора на експресију и деловање неуроендокриних регулатора енергетске хомеостазе (RS-MESTD-Basic Research (BR or ON)-173023)
У:
- European Journal of Neuroscience (2017), 46(1): 1730-1737
DOI: 10.1111/ejn.13607
ISSN: 953-816X
PubMed: 28544147