Edible wild plant Heracleum pyrenaicum subsp. orsinii as a potential new source of bioactive essential oils
2017
Authors:
Ušjak, LjubošPetrović, Silvana
Drobac, Milica
Soković, Marina
Stanojković, Tatjana
Ćirić, Ana
Niketić, Marjan
Document Type:
Article (Published version)
,
© Association of Food Scientists & Technologists (India) 2017
Metadata
Show full item recordAbstract:
Many Heracleum L. taxa (Apiaceae) are used as food and spices, and in traditional medicine. In this work, the chemical composition of Heracleum pyrenaicum subsp. orsinii (Guss.) F. Pedrotti and Pignatti root, leaf and fruit essential oils, their antimicrobial activity and cytotoxic effect on malignant and normal cells were investigated for the first time. The composition of the oils was analyzed by GC and GC–MS. Monoterpenes prevailed in the root oil, with β-pinene (38.6%) being dominant, while in the leaf oil, sesquiterpenes, mostly (E)-nerolidol (20.5%) and (E)-caryophyllene (17.0%), were the most abundant constituents. The fruit oil contained the majority of aliphatic esters, mainly octyl acetate (36.8%) and octyl hexanoate (22.1%). The antimicrobial activity was determined by microdilution method against eight bacteria and eight fungi (standard strains, clinical or food isolates). The best antibacterial activity, better than the activity of ampicillin, was shown by the root oil against Salmonella typhimurium, Escherichia coli and Pseudomonas aeruginosa. The strongest antifungal activity, stronger than the activity of ketoconazole, was exhibited by the leaf and root oils against Trichoderma viride, and by the root oil against Aspergillus ochraceus. The cytotoxic effect of the oils, determined by MTT test, was prominent against malignant HeLa, LS174 and A549 cells (IC50 = 6.49–14.56 μg/mL). On the other hand, the oils did not show toxicity against normal MRC-5 cells at tested concentrations (IC50 > 200.00 μg/mL). It can be concluded that investigated H. pyrenaicum subsp. orsinii oils represent potential new raw materials for food and pharmaceutical industry.
Keywords:
Heracleum pyrenaicum subsp. orsinii; Essential oils; Chemical composition; Antimicrobial activity; Cytotoxic effectFunding / projects:
- Investigation on the medicinal plants: morphological, chemical and pharmacological characterisation (RS-MESTD-Basic Research (BR or ON)-173021)
- Characterization and application of fungal metabolites and assessment of new biofungicides potential (RS-MESTD-Basic Research (BR or ON)-173032)
- Biological response modifiers in physiological and pathological conditions (RS-MESTD-Basic Research (BR or ON)-175011)
In:
- Journal of Food Science and Technology (2017), 54(8): 2193-2202
DOI: 10.1007/s13197-017-2610-z
ISSN: 0022-1155
WoS: 000405272300001
Scopus: 2-s2.0-85020530697
URI
http://link.springer.com/10.1007/s13197-017-2610-zhttps://radar.ibiss.bg.ac.rs/handle/123456789/2784