Testiranje različitih metoda uzorkovanja makrobeskičmenjaka u vodenim ekosistemima i mogućnosti standardizacije
Testing different methods for sampling macroinvertebrates in aquatic ecosystems and examination of the possibility for their standardization
Abstract:
Aquatic macroinvertebrate communities possess certain characteristics which render
them as reliable indicators of environmental changes in aquatic ecosystems. In the review of
the application of the Water Framework Directive in European countries, it was observed that
in most cases water macroinvertebrates serve as the significant typological element, and that
the reference conditions are based mainly on the researched group of hydro-biota.
Current research in Europe is aimed at reviewing and establishing practical methods
for sampling macroinvertebrates in order to ensure the successful implementation of the
Water Framework Directive. Field work is the first step in every analysis of an
macroinvertebrate community and the choice of efficient methods with regard to the type of
ecosystem and goals of the study is of a great importance. Insufficient financial and technical resources for monitoring and applied research require the establishment of an effective
methodology for collecting biological samples and a harmonized approach to insure data
comparability. Applied research depends on the available financial resources and the
scientific objective.
This paper presents different sampling techniques for the study of aquatic
macroinvertebrate communities. It examines the problems encountered during sampling of
specific faunistic ecological groups and different types of water bodies. The material was
collected at 320 sites located primarily along different running waters in Serbia, from 2004 to
2016. To establish the efficiency of a particular method, we used data that was collected at
sites outside of Serbia as follows: (i) the Danube River (a 2,500 km long sector from
Regensburg to the Danube Delta); (ii) the Sava River (the entire course of the river with a
length of 937 km); (iii) the rivers Neretva and Bosna in Bosnia and Herzegovina, including
their tributaries.
A detailed protocol for collecting material and supporting data is presented, including
description of aquatic macroinvertebrate sampling techniques, selection and characterization
of the sites, reviews of procedures used for the processing of the sampled material, as well as
a discussion of the methods employed for the analysis of the obtained results.
The results were analyzed with the software package ASTERICS 4.0.4 (AQEM
Consortium, 2002) and "Statistica" Version 7 (StatSoft, Inc., 2004). Comparison of the
different sampling methods for aquatic macroinvertebrate communities and specific groups
within communities provided answers with regard to the standardization of the
methodologies, the effects of the individual sampling methods on the results related to the
diversity and spatial dynamics of the aquatic macroinvertebrate communities in different
aquatic ecosystems.
Based on the obtained results, we optimized the aquatic macroinvertebrate sampling
methodology for the aquatic ecosystems in Serbia. Also, methodologies for specific types of
ecosystems and appropriate methods of selection for specific types of research are suggested,
as well as methodologies best suited for a specific group of organisms (Mussels), with
emphasis on large and very large rivers (based on the National typology of running waters in
Serbia) which represent complex ecosystems.
Keywords:
Aquatic macroinvertebrates; Sampling technique; Sampling efficiency; Standardization; Study designSource:
University of Kragujevac, Faculty of Science, 2016, 1-130Funding / projects:
- Evolution in Heterogeneous Environments: Adaptation Mechanisms, Biomonitoring and Conservation of Biodiversity (RS-MESTD-Basic Research (BR or ON)-173025)
- Monitoring and Modeling of Rivers and Reservoirs (MORE) - Physical, Chemical, Biological and Morphodynamic Parameters (RS-MESTD-Technological Development (TD or TR)-37009)
URI
http://eteze.kg.ac.rs/application/showtheses?thesesId=4605https://fedorakg.kg.ac.rs/fedora/get/o:750/bdef:Content/download
http://nardus.mpn.gov.rs/123456789/7628
https://radar.ibiss.bg.ac.rs/handle/123456789/2813