Anti-encephalitogenic effects of cucumber leaf extract
2017
Authors:
Jevtić, BojanNikolovski, Neda
Stanisavljević, Suzana
Gašić, Uroš
Mišić, Danijela
Despotović, Jovana
Samardžić, Jelena
Miljković, Đorđe
Timotijević, Gordana
Document Type:
Article (Published version)
,
© 2017 Elsevier Ltd.
Metadata
Show full item recordAbstract:
Cucumber (Cucumis sativus) fruit has been used in cuisine worldwide, while its leaves are rich in immunomodulatory compounds. Cucumber leaf extract (CLE) was characterized by the predominance of triterpenoids cucurbitacins and significant levels of phenolics. Effects of CLE on CD4 + T helper (Th) cells and macrophages, as the major encephalitogenic cells in the autoimmunity of the central nervous system were investigated in our study. CLE potently inhibited production of major pathogenic Th cytokines: interferon-gamma and interleukin-17, as well as of nitric oxide and reactive oxygen species in macrophages. Antigen-presenting activity of macrophages and dendritic cells was also affected by CLE. The effects of CLE were co-incident with modulation of NFκB and p38 MAPK signaling. Concentrations of CLE used in vitro did not show toxic effects on zebrafish embryos. Moreover, CLE inhibited generation of encephalitogenic cells in vivo. These results demonstrate that CLE deserve further investigation on its anti-encephalitogenic therapeutic properties.
Keywords:
Cucumber; Cucurbitacin; T cell; Macrophage; Autoimmunity; Experimental autoimmune encephalomyelitisFunding / projects:
- Structure-properties relationships of natural and synthetic molecules and their metal complexes (RS-MESTD-Basic Research (BR or ON)-172017)
- The Role of Transcription Factors and Small RNAs in Abiotic Stress Response in Plants and Genetic Diversity of Plant Species Important for Agriculture and Biotechnology (RS-MESTD-Basic Research (BR or ON)-173005)
- Complex diseases as a model system for phenotype modulation- structural and functional analysis of molecular biomarkers (RS-MESTD-Basic Research (BR or ON)-173008)
- Molecular mechanisms of physiological and pharmacological control of inflammation and cancer (RS-MESTD-Basic Research (BR or ON)-173013)
- Physiological, chemical and molecular analysis of the diversity of selected rare and endangered plant species and application of biotechnology for ex situ conservation and production of biologically active compounds (RS-MESTD-Basic Research (BR or ON)-173024)
- Cellular and molecular mechanisms of recovery of rats from experimental autoimmune encephalomyelitis (RS-MESTD-Basic Research (BR or ON)-173035)
In:
- Journal of Functional Foods (2017), 37: 249-262
DOI: 10.1016/j.jff.2017.07.060
ISSN: 1756-4646
WoS: 000412863400025
Scopus: 2-s2.0-85026788295
URI
http://linkinghub.elsevier.com/retrieve/pii/S1756464617304486https://radar.ibiss.bg.ac.rs/handle/123456789/2832