Sex as a determinant of age-related changes in rat spinal cord inflammation-oxidation state
2017
Authors:
Nacka-Aleksić, MirjanaStojanović, Marija
Simić, Lidija
Bufan, Biljana
Kotur-Stevuljević, Jelena
Stojić-Vukanić, Zorica
Dimitrijević, Mirjana
Ražić, Slavica
Leposavić, Gordana
Document Type:
Article (Accepted Version)
,
© 2017 Springer Science+Business Media B.V.
Metadata
Show full item recordAbstract:
To close the gap in our knowledge of sex influence on age-related changes in inflammation-oxidation state in spinal cord (SC) relevant to inflammation/oxidative-stress associated neuropathologies, 2–3 month-old (young) and 18–20 month-old (old) rats, exhibiting increased level of IL-6, a commonly used marker of inflamm-aging, were examined for inflammatory/redox status, and the underlying regulatory networks’ molecules expression. With age, rat SC microglia became sensitized (“primed”), while SC tissue shifted towards mild inflammatory state, with increased levels of proinflammatory IL-1β (key marker of microglial systemic inflammation-induced neurotoxicity), which was more prominent in males. This, most likely, reflected age- and sex-related impairment in the expression of CX3CR1, the receptor for fractalkine (CX3CL1), the soluble factor which regulates microglial activation and diminishes production of IL-1β (central for fractalkine neuroprotection). Considering that (i) age-related changes in SC IL-1β expression were not followed by complementary changes in SC IL-6 expression, and (ii) the reversal in the direction of the sex bias in circulating IL-6 level and SC IL-1β expression, it seems obvious that there are tissue-specific differences in the proinflammatory cytokine profile. Additionally, old male rat SC exhibited greater oxidative damage than female, reflecting, most likely, their lower capacity to maintain the pro-oxidant-antioxidant balance. In conclusion, these findings, apart from highlighting the significance of sex for age-associated changes in SC inflammation-oxidation, may be relevant for understating sex differences in human inflammation/oxidative-stress related SC diseases, and consequently, for optimizing their prevention/therapy.
Keywords:
Aging; Spinal cord; Inflammatory state; Oxidation state; CX3CR1Source:
Biogerontology, 2017Funding / projects:
- Immune system plasticity during aging: Immunomodulatory capacity of oestrogens (RS-MESTD-Basic Research (BR or ON)-175050)
DOI: 10.1007/s10522-017-9726-4
ISSN: 1389-5729
PubMed: 28825141
WoS: 000410707700008
Scopus: 2-s2.0-85027852656
URI
http://link.springer.com/10.1007/s10522-017-9726-4https://radar.ibiss.bg.ac.rs/handle/123456789/2838