A single high dose of dexamethasone increases GAP-43 and synaptophysin in the hippocampus of aged rats
2017
Аутори:
Tešić, VesnaPerović, Milka
Zaletel, Ivan
Jovanović, Mirna
Puškaš, Nela
Ruždijić, Sabera
Kanazir, Selma
Тип документа:
Чланак у часопису (Објављена верзија)
,
© 2017 Elsevier Inc.
Метаподаци
Приказ свих података о документуАпстракт:
The administration of dexamethasone, a synthetic glucocorticoid receptor agonist, has been reported to modulate cognitive performance in both animals and humans. In the present study, we demonstrate the effects of a single high dose of dexamethasone on the expression and distribution of synaptic plasticity-related proteins, growth-associated protein-43 (GAP-43) and synaptophysin, in the hippocampus of 6-, 12-, 18- and 24-month-old rats. Acute dexamethasone treatment significantly altered the expression of GAP-43 at the posttranslational level by modulating the levels of phosphorylated GAP-43 and proteolytic GAP-43-3 fragment. The effect was the most pronounced in the hippocampi of the aged animals. The total GAP-43 protein was increased only in 24-month-old dexamethasone-treated animals, and was concomitant with a decrease in calpain-mediated proteolysis. Moreover, by introducing the gray level co-occurrence matrix method, a form of texture analysis, we were able to reveal the subtle differences in the expression pattern of both GAP-43 and synaptophysin in the hippocampal subfields that were not detected by Western blot analysis alone. Therefore, the current study demonstrates, through a novel combined approach, that dexamethasone treatment significantly affects both GAP-43 and synaptophysin protein expression in the hippocampus of aged rats.
Кључне речи:
Aging; GAP-43 cleavage; GAP-43 phosphorylation; GLCM texture analysis; Hippocampus; αII-spectrin cleavageИзвор:
Experimental Gerontology, 2017, 98, 62-69Финансирање / пројекти:
- Пластичност мозга током старења: утицај дијеталне рестрикције и анестезије (RS-MESTD-Basic Research (BR or ON)-173056)
DOI: 10.1016/j.exger.2017.08.010
ISSN: 0531-5565
PubMed: 28801169
WoS: 000415375500009
Scopus: 2-s2.0-85027505496
URI
http://linkinghub.elsevier.com/retrieve/pii/S0531556517302462https://radar.ibiss.bg.ac.rs/handle/123456789/2839