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A single high dose of dexamethasone increases GAP-43 and synaptophysin in the hippocampus of aged rats
dc.creator | Tešić, Vesna | |
dc.creator | Perović, Milka | |
dc.creator | Zaletel, Ivan | |
dc.creator | Jovanović, Mirna | |
dc.creator | Puškaš, Nela | |
dc.creator | Ruždijić, Sabera | |
dc.creator | Kanazir, Selma | |
dc.date.accessioned | 2017-11-23T11:32:12Z | |
dc.date.available | 2900-01-01 | |
dc.date.issued | 2017 | |
dc.identifier.issn | 0531-5565 | |
dc.identifier.uri | http://linkinghub.elsevier.com/retrieve/pii/S0531556517302462 | |
dc.identifier.uri | https://radar.ibiss.bg.ac.rs/handle/123456789/2839 | |
dc.description.abstract | The administration of dexamethasone, a synthetic glucocorticoid receptor agonist, has been reported to modulate cognitive performance in both animals and humans. In the present study, we demonstrate the effects of a single high dose of dexamethasone on the expression and distribution of synaptic plasticity-related proteins, growth-associated protein-43 (GAP-43) and synaptophysin, in the hippocampus of 6-, 12-, 18- and 24-month-old rats. Acute dexamethasone treatment significantly altered the expression of GAP-43 at the posttranslational level by modulating the levels of phosphorylated GAP-43 and proteolytic GAP-43-3 fragment. The effect was the most pronounced in the hippocampi of the aged animals. The total GAP-43 protein was increased only in 24-month-old dexamethasone-treated animals, and was concomitant with a decrease in calpain-mediated proteolysis. Moreover, by introducing the gray level co-occurrence matrix method, a form of texture analysis, we were able to reveal the subtle differences in the expression pattern of both GAP-43 and synaptophysin in the hippocampal subfields that were not detected by Western blot analysis alone. Therefore, the current study demonstrates, through a novel combined approach, that dexamethasone treatment significantly affects both GAP-43 and synaptophysin protein expression in the hippocampus of aged rats. | en |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173056/RS// | |
dc.rights | restrictedAccess | |
dc.source | Experimental Gerontology | |
dc.subject | Aging | |
dc.subject | GAP-43 cleavage | |
dc.subject | GAP-43 phosphorylation | |
dc.subject | GLCM texture analysis | |
dc.subject | Hippocampus | |
dc.subject | αII-spectrin cleavage | |
dc.title | A single high dose of dexamethasone increases GAP-43 and synaptophysin in the hippocampus of aged rats | en |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Перовић, Милка; Каназир, Селма; Руждијић, Сабера; Пушкаш, Нела; Залетел, Иван; Тешић, Весна; Јовановић, Мирна; | |
dc.rights.holder | © 2017 Elsevier Inc. | |
dc.citation.volume | 98 | |
dc.identifier.doi | 10.1016/j.exger.2017.08.010 | |
dc.identifier.pmid | 28801169 | |
dc.identifier.scopus | 2-s2.0-85027505496 | |
dc.identifier.wos | 000415375500009 | |
dc.citation.apa | Tesic, V., Perovic, M., Zaletel, I., Jovanovic, M., Puskas, N., Ruzdijic, S., & Kanazir, S. (2017). A single high dose of dexamethasone increases GAP-43 and synaptophysin in the hippocampus of aged rats. Experimental Gerontology, 98, 62–69. | |
dc.citation.vancouver | Tesic V, Perovic M, Zaletel I, Jovanovic M, Puskas N, Ruzdijic S, Kanazir S. A single high dose of dexamethasone increases GAP-43 and synaptophysin in the hippocampus of aged rats. Exp Gerontol. 2017;98:62–9. | |
dc.citation.spage | 62 | |
dc.citation.epage | 69 | |
dc.type.version | publishedVersion | en |
dc.citation.rank | M21 |