Strain-specific helper T cell profile in the gut-associated lymphoid tissue
2017
Authors:
Stanisavljević, SuzanaNikolovski, Neda
Vujičić, Milica
Saksida, Tamara
Jevtić, Bojan
Milovanović, Boško
Momčilović, Miljana
Miljković, Đorđe
Stojanović, Ivana D.
Document Type:
Article (Published version)
,
© 2017 European Federation of Immunological Societies
Metadata
Show full item recordAbstract:
C57BL/6, BALB/c and NOD mice are among the most frequently used strains in autoimmunity research. NOD mice spontaneously develop type 1 diabetes (T1D) and they are prone to induction of experimental autoimmune encephalomyelitis (EAE). Both diseases can be routinely induced in C57BL/6 mice, but not in BALB/c mice. Also, C57BL/6 mice are generally considered T helper (Th)1-biased and BALB/c Th2-biased mice. Having in mind increasingly appreciated role of gut associated lymphoid tissue (GALT) cells in autoimmunity, especially in relation to gut Th17 and regulatory T (Treg) cells, our aim was to determine if there are differences in proportion of CD4 + T cell populations in mesenteric lymph nodes and Peyer's p atches of these mouse strains. Lower proportion of Treg was observed in NOD PP, Th2 cells dominated in BALB/c mice in mesenteric lymph nodes (MLN) and Peyer's patches (PP), while Th1 cells prevailed in C57BL/6 MLN. Intradermal immunization of mice with complete Freund's adjuvant resulted in significant difference in Th cell distribution in GALT of NOD mice. Differences were less pronounced in C57BL/6 mice, while GALT of BALB/c mice was almost unresponsive to the immunization. The observed strain- and tissue-dependent changes in Treg proportion after the immunization was probably a consequence of different CCR2 or CCR6-related migration patterns and/or in situ Treg proliferation. In conclusion, NOD, a highly autoimmunity-prone mouse strain, exhibits more profound GALT-related immune response upon immunization compared to the strains that are less prone to autoimmunity.
Keywords:
Type 1 diabetes; Multiple sclerosis; Autoimmunity; Gut-associated lymphoid tissue; ImmunizationFunding / projects:
- Molecular mechanisms of physiological and pharmacological control of inflammation and cancer (RS-MESTD-Basic Research (BR or ON)-173013)
- Cellular and molecular mechanisms of recovery of rats from experimental autoimmune encephalomyelitis (RS-MESTD-Basic Research (BR or ON)-173035)
In:
- Immunology Letters (2017), 190: 282-288
DOI: 10.1016/j.imlet.2017.08.017
ISSN: 165-2478
PubMed: 28851631
WoS: 000412963500035
Scopus: 2-s2.0-85029008926
URI
http://linkinghub.elsevier.com/retrieve/pii/S0165247817301979https://radar.ibiss.bg.ac.rs/handle/123456789/2854