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Chlamydia trachomatis infection and development of epithelial mesenchymal transition in conjunctiva : possible epigenetic mechanisms unveiled
dc.contributor.editor | Brajušković, Goran | |
dc.contributor.editor | Đorđević, Ana | |
dc.creator | Rajić, Jovana | |
dc.creator | Grdović, Nevena | |
dc.creator | Inic-Kanada, Aleksandra | |
dc.creator | Stein, Elisabeth | |
dc.creator | Schuerer, Nadine | |
dc.creator | Uskoković, Aleksandra | |
dc.creator | Ghasemian, Ehsan | |
dc.creator | Dinić, Svetlana | |
dc.creator | Mihailović, Mirjana | |
dc.creator | Arambašić Jovanović, Jelena | |
dc.creator | Tolić, Anja | |
dc.creator | Đorđević, Marija | |
dc.creator | Đorđević, Miloš | |
dc.creator | Poznanović, Goran | |
dc.creator | Barisani-Asenbauer, Talin | |
dc.creator | Vidaković, Melita | |
dc.date.accessioned | 2017-11-23T08:34:40Z | |
dc.date.available | 2017-11-23T08:34:40Z | |
dc.date.issued | 2017 | |
dc.identifier.uri | https://radar.ibiss.bg.ac.rs/handle/123456789/2869 | |
dc.identifier.uri | https://radar.ibiss.bg.ac.rs/handle/123456789/2866 | |
dc.description.abstract | Introduction: Trachoma is the most common cause of infectious blindness worldwide, initiated by repeated infection of the conjunctiva with Chlamydia trachomatis (Ct). The resulting chronic inflammation and formation of fibrotic tissue eventually lead to corneal damage. Based on the facts that epithelial to mesenchymal transition (EMT) plays an important role in the development of fibrosis and that EMT is epigenetically regulated process, the aims of this study were to reveal the capacity of Ct to induce EMT in vitro and to unveil potential underlying epigenetic mechanisms. Methods: Human conjunctival epithelial (HCjE) cells were infected with 107 IFU of Ct for 72 h. EMT-inducing signaling pathways, as well as mRNA and protein expression of EMT markers (E-cadherin, fibronectin and α-SMA) were evaluated by RT-qPCR, Immunoblotting and Immunocytochemistry. DNA methylation patterns of selected regions of E-cadherin, fibronectin and α-SMA genes were examined by Methylation-Specific PCR, High Resolution Melting analysis and Bisulfite Sequencing. Results: Infection with Ct was accompanied with the activation of EMT-inducing signaling pathways, downregulation of epithelial marker E-cadherin and upregulation of mesenchymal markers fibronectin and α-SMA. While DNA methylation status of E-cadherin gene promoter correlated with its expression, methylation status of fibronectin and α-SMA genes couldn’t be related to their expression levels. Conclusion: Ct infection of HCjE cells triggers EMT that goes along with changes in the methylation profile of the E-cadherin promoter. Sequence of events described herein could contribute to scarring process in trachoma and open up possibilities for development of new therapeutic strategies in trachoma treatment. | en |
dc.publisher | Belgrade: University of Belgrade, Faculty of Biology | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173020/RS// | |
dc.relation | “Laura Bassi Centers of Expertise” program of the Austrian Federal Ministry of Economy through the Austrian Research Promotion Agency (FFG project number 822768) | |
dc.relation.isversionof | https://radar.ibiss.bg.ac.rs/handle/123456789/2866 | |
dc.rights | openAccess | |
dc.source | 1st Congress of Molecular Biologists of Serbia | |
dc.title | Chlamydia trachomatis infection and development of epithelial mesenchymal transition in conjunctiva : possible epigenetic mechanisms unveiled | en |
dc.type | conferenceObject | |
dc.rights.license | ARR | |
dcterms.abstract | Рајић, Јована; Грдовић, Невена; Арамбашић Јовановић, Јелена; Михаиловић, Мирјана; Толић, Ања; Видаковић, Мелита; Ускоковић, Александра; Динић, Светлана; Синадиновић, Марија; Ђорђевић, Милош; Познановић, Горан; Ђорђевић, Ана; Иниц-Канада, Александра; Стеин, Елисабетх; Сцхуерер, Надине; Гхасемиан, Ехсан; Барисани-Асенбауер, Талин; Брајушковић, Горан | |
dc.rights.holder | © University of Belgrade, Faculty of Biology | |
dc.description.other | Brajušković G, Đorđević A, editors. CoMBoS. 1st Congress of Molecular Biologists of Serbia; 2017 Sep 20-22; Belgrade, Serbia. Belgrade: University of Belgrade, Faculty of Biology; 2017. p. 70. | |
dc.citation.apa | Rajić, J., Grdović, N., Inic-kanada, A., Stein, E., Schuerer, N., Uskoković, A., Ghasemian, E., et al. (2017). Chlamydia trachomatis infection and development of epithelial mesenchymal transition in conjunctiva : possible epigenetic mechanisms unveiled. In G. Brajušković & A. Đorđević (Eds.), 1st Congress of Molecular Biologists of Serbia. Belgrade: University of Belgrade, Faculty of Biology (p. 70). | |
dc.citation.vancouver | Rajić J, Grdović N, Inic-kanada A, Stein E, Schuerer N, Uskoković A, Ghasemian E, Dinić S, Mihailović M, Arambašić Jovanović J, Tolić A, Sinadinović M, Đorđević M, Poznanović G, Barisani-Asenbauer T, Vidaković M. Chlamydia trachomatis infection and development of epithelial mesenchymal transition in conjunctiva : possible epigenetic mechanisms unveiled. In: Brajušković G, Đorđević A, editors. CoMBoS. 1st Congress of Molecular Biologists of Serbia; 2017 Sep 20-22; Belgrade, Serbia. Belgrade: University of Belgrade, Faculty of Biology; 2017. p. 70. | |
dc.citation.spage | 70 | |
dc.citation.epage | 70 | |
dc.type.version | publishedVersion | en |
dc.identifier.fulltext | https://radar.ibiss.bg.ac.rs/bitstream/id/455/bitstream_455.pdf | |
dc.identifier.rcub | https://hdl.handle.net/21.15107/rcub_ibiss_2869 |