Antimicrobial synergism and cytotoxic properties of Citrus limon L., Piper nigrum L. and Melaleuca alternifolia (Maiden and Betche) Cheel essential oils
2017
Autori:
Nikolić, MilošJovanović, Katarina
Marković, Tatjana
Marković, Dejan
Gligorijević, Nevenka
Radulović, Siniša
Kostić, Marina
Glamočlija, Jasmina
Soković, Marina
Tip dokumenta:
Članak u časopisu (Objavljena verzija)
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© 2017 Royal Pharmaceutical Society
Metapodaci
Prikaz svih podataka o dokumentuApstrakt:
Objectives: The chemical composition, antimicrobial and synergistic effect, and cytotoxic activity of Citrus limon (lemon), Piper nigrum (green pepper) and Melaleuca alternifoila (tea tree) essential oils (EOs) were investigated. Methods: Chemical analyses of essential oils were tested by GC-FID and GC-MS spectroscopy. The antimicrobial activity assay was conducted using microdilution method against several oral bacteria and Candida spp. originating from the humans with oral disorders. The synergistic antimicrobial activity was evaluated using checkerboard method. The cytotoxicity evaluation of EOs was assessed using MTT test. Key findings: Limonene (37.5%) and β-pinene (17.9%) were the major compounds in C. limon oil, β-pinene (34.4%), δ-3-carene (19.7%), limonene (18.7%) and α-pinene (10.4%) in P. nigrum oil and terpinen-4-ol (38.6%) and γ-terpinene (21.7%) in M. alternifolia oil. The broad-spectrum antimicrobial activity was achieved by tested three EOs, with C. limon oil being the strongest against bacteria and M. alternifolia oil strongest against fungi. The EOs demonstrated synergism; their combined application revealed an increase in antimicrobial activity. All tested essential oils showed lower cytotoxic activity in comparison with the positive control, and the obtained results confirmed a dose-dependent activity. Conclusions: The results of this study encourage use of tested EOs in development of a novel agent intended for prevention or therapy of corresponding oral disorders.
Ključne reči:
antimicrobial synergism; Citrus limon; Cytotoxic activity; Essential oil; Melaleuca alternifolia; Piper nigrumFinansiranje / projekti:
- Karakterizacija i primena metabolita gljiva i utvrđivanje potencijala novih biofungicida (RS-MESTD-Basic Research (BR or ON)-173032)
- Farmakodinamska i farmakogenomska ispitivanja novijih lekova u lečenju solidnih tumora (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41026)
U:
- Journal of Pharmacy and Pharmacology (2017), 69(11): 1606-1614
DOI: 10.1111/jphp.12792
ISSN: 0022-3573
PubMed: 28815601