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CXC chemokine ligand 12α-mediated increase in insulin secretion and survival of mouse pancreatic islets in response to oxidative stress through modulation of calcium uptake
dc.creator | Vidaković, Melita | |
dc.creator | Mihailović, Mirjana | |
dc.creator | Đorđević, Marija | |
dc.creator | Rajić, Jovana | |
dc.creator | Uskoković, Aleksandra | |
dc.creator | Dinić, Svetlana | |
dc.creator | Grdović, Nevena | |
dc.creator | Đorđević, Miloš | |
dc.creator | Tolić, Anja | |
dc.creator | Poznanović, Goran | |
dc.creator | Caballero, Garrido | |
dc.creator | Arambašić Jovanović, Jelena | |
dc.date.accessioned | 2017-11-23T07:56:40Z | |
dc.date.available | 2017-11-23T07:56:40Z | |
dc.date.issued | 2018 | |
dc.identifier.issn | 0354-4664 | |
dc.identifier.uri | http://www.doiserbia.nb.rs/Article.aspx?ID=0354-46641700040V | |
dc.identifier.uri | https://radar.ibiss.bg.ac.rs/handle/123456789/2886 | |
dc.description.abstract | We examined whether CXCL12α improves insulin secretion by influencing the Ca2+ oscillation pattern and Ca2+ influx ([Ca2+]i), thereby enhancing the viability of pancreatic islet cells in oxidative stress. The islets of Langerhans were isolated from male OF1 mice and pretreated with 40 ng/mL of CXCL12α prior to exposure to 7.5 μM hydrogen peroxide, which served to induce oxidative stress. Incubation of islets with CXCL12α induced pancreatic β-cell proliferation and improved the ability of β-cells to withstand oxidative stress. Consecutive treatments of isolated islets with hydrogen peroxide caused a decline in β-cell functioning over time, while the CXCL12α pretreatment of islets exhibited a physiological response to high glucose that was comparable to control islets. The attenuated response of islets to a high D-glucose challenge was observed as a partial to complete abolishment of [Ca2+]i. Treatments with increasing concentrations of CXCL12α decreased the number of Ca2+ oscillations that lasted longer, thus pointing to an overall increase in [Ca2+]i, which was followed by increased insulin secretion. In addition, treatment of islets with CXCL12α enhanced the transcription rate for insulin and the CXCR4 gene, pointing to the importance of CXCL12/CXCR4 signaling in the regulation of Ca2+ intake and insulin secretion in pancreatic islet cells. We propose that a potential treatment with CXCL12α could help to remove preexisting glucotoxicity and associated temporary β-cell stunning that might be present at the time of diabetes diagnosis in vivo. | en |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173020/RS// | |
dc.relation.isversionof | https://radar.ibiss.bg.ac.rs/handle/123456789/3023 | |
dc.rights | openAccess | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | |
dc.source | Archives of Biological Sciences | |
dc.subject | Diabetes | |
dc.subject | Calcium | |
dc.subject | CXC chemokine ligand 12α | |
dc.subject | Insulin | |
dc.subject | Pancreatic islet cells | |
dc.subject | Voltage-gated calcium channels | |
dc.title | CXC chemokine ligand 12α-mediated increase in insulin secretion and survival of mouse pancreatic islets in response to oxidative stress through modulation of calcium uptake | en |
dc.type | article | |
dc.rights.license | BY-NC-ND | |
dcterms.abstract | Арамбашић Јовановић, Јелена; Рајић, Јована; Видаковић, Мелита; Цабаллеро, Гарридо; Михаиловић, Мирјана; Синадиновић, Марија; Ускоковић, Александра; Динић, Светлана; Грдовић, Невена; Ђорђевић, Милош; Толић, Ања; Познановић, Горан | |
dc.rights.holder | © Serbian Biological Society | |
dc.citation.issue | 1 | |
dc.citation.volume | 70 | |
dc.identifier.doi | 10.2298/ABS170711040V | |
dc.identifier.scopus | 2-s2.0-85043718185 | |
dc.identifier.wos | 000428370100019 | |
dc.citation.apa | Vidaković, M., Caballero, G., Mihailović, M., Arambašić-Jovanović, J., Sinadinović, M., Rajić, J., Uskoković, A., et al. (2018). CXC chemokine ligand 12α-mediated increase in insulin secretion and survival of mouse pancreatic islets in response to oxidative stress through modulation of calcium uptake. Archives of Biological Sciences, 70(1), 191–204. | |
dc.citation.vancouver | Vidaković M, Caballero G, Mihailović M, Arambašić-Jovanović J, Sinadinović M, Rajić J, Uskoković A, Dinić S, Grdović N, Đorđević M, Tolić A, Poznanović G. CXC chemokine ligand 12α-mediated increase in insulin secretion and survival of mouse pancreatic islets in response to oxidative stress through modulation of calcium uptake. Arch Biol Sci. 2018;70(1):191–204. | |
dc.citation.spage | 191 | |
dc.citation.epage | 204 | |
dc.type.version | acceptedVersion | |
dc.identifier.fulltext | https://radar.ibiss.bg.ac.rs/bitstream/id/79/bitstream_79.pdf | |
dc.citation.rank | M23 |