Evaluation of genotoxic potential in the Velika Morava River Basin in vitro and in situ
2018
Authors:
Jovanović, JovanaKolarević, Stoimir
Milošković, Aleksandra
Radojković, Nataša
Simić, Vladica
Dojčinović, Biljana
Kračun-Kolarević, Margareta
Paunović, Momir
Kostić, Jovana
Sunjog, Karolina
Gačić, Zoran
Vuković-Gačić, Branka
Timilijić, Jovana
Djordjević, Jelena
Žegura, Bojana
Document Type:
Article (Accepted Version)
,
© 2017 Elsevier B.V.
Metadata
Show full item recordAbstract:
The Velika Morava River is the greatest national Serbian river and the significant tributary of the Danube River. The major problems in the Velika Morava River Basin (VMRB) represent untreated industrial and municipal wastewaters. In this study, the level of genotoxic potential at the sites along the VMRB was evaluated by parallel in vitro and in situ approach. Within in vitro testing, genotoxicity of native water samples collected from the sites in VMRB was evaluated by SOS/umuC test on Salmonella typhimurium TA1535/pSK1002 and by the comet assay on HepG2 cells. DNA damage in situ was assessed in bleak (Alburnus alburnus) erythrocytes by the comet (alkaline and Fpg-modified comet) and micronucleus assays. Additionally, the concentration of heavy metals in fish tissue was measured and this data, compiled with the data of the physico-chemical parameters measured in water, was used as a measure of the pollution pressure at the sites. Results showed that applied in vitro tests with native water samples are less sensitive in comparison with in situ tests and should be taken with precaution when making predictions on the status of the ecosystem. Within applied battery of in situ assays differential sensitivity of assays was observed where alkaline comet assay showed the highest potential in differentiation of the sites based on genotoxic potential. Integrated biomarker response showed that usage of the battery of bioassays provides better insight in a genotoxic effects in animals, and consequently, that the holistic approach is more suitable for this type of study.
Keywords:
Eco/genotoxicity; SOS/umuC; HepG2 cells; Comet assay; The Velika Morava River; Alburnus alburnusSource:
Science of The Total Environment, 2018, 621, 1289-1299Funding / projects:
- The influence of the quality of the components of food for cyprinid fish species on the quality of meat, losses and the profitability of production (RS-MESTD-Technological Development (TD or TR)-31011)
- Monitoring and Modeling of Rivers and Reservoirs (MORE) - Physical, Chemical, Biological and Morphodynamic Parameters (RS-MESTD-Technological Development (TD or TR)-37009)
- Fishes as water quality indicators in open waters of Serbia (RS-MESTD-Basic Research (BR or ON)-173045)
DOI: 10.1016/j.scitotenv.2017.10.099
ISSN: 0048-9697; 0048-9697
PubMed: 29056382
WoS: 000424196800127
Scopus: 2-s2.0-85031791196
URI
http://linkinghub.elsevier.com/retrieve/pii/S004896971732805Xhttps://radar.ibiss.bg.ac.rs/handle/123456789/2887