In vitro and in vivo transformations of Centaurium erythraea secoiridoid glucosides alternate their antioxidant and antimicrobial capacity
2018
Autori:
Božunović, JelenaŽivković, Suzana
Gašić, Uroš
Glamočlija, Jasmina
Ćirić, Ana
Matekalo, Dragana
Šiler, Branislav
Soković, Marina
Tešić, Živoslav
Mišić, Danijela
Tip dokumenta:
Članak u časopisu (Objavljena verzija)
,
© 2017 Elsevier B.V.
Metapodaci
Prikaz svih podataka o dokumentuApstrakt:
The present study was principally aimed at ascertaining the differences in metabolomics profiles and biological activities between non-hydrolyzed (ME) and hydrolyzed methanol extract (HME) of Centaurium erythraea Rafn. UHPLC–MS/MS Orbitrap analysis showed that the enzymatic hydrolysis of the extract caused changes in β-d-glycoside/aglycone ratio of both flavonoid and secoiridoid compounds. UHPLC/DAD/+HESI−qqqMS characterization and/or quantification of secoiridoid glucosides (SGs) and their aglycones in both ME and HME revealed gentiopicral and erythrocentaurin as the major aglycones, the same metabolic products which appear after the hydrolysis of pure swertiamarin, the dominant secoiridoid glucoside of C. erythraea. SGs played an antioxidant role only in ABTS assay, whilst the remarkable antioxidant potential of C. erythraea methanol extract is ascribed chiefly to phenolics detected in it. Interestingly, antioxidant activities of swertiamarin and sweroside recorded in ABTS assay increased after the compounds have been hydrolyzed, which highlighted their possible antioxidant role during ingestion. Strong antimicrobial activities of ME and HME against a vast array of pathogens, which exceed the effects of the reference antibiotics and antimycotics, largely depended on the amount of secoiridoids in either of the glycosylation forms. Extracts and pure secoiridoids were especially effective against most of the tested Penicillium species. On the other hand, P. funiculosum has evolved an efficient mechanism of detoxification of sub-lethal concentrations of secoiridoid glucosides, involving their biotransformation and complete digestion. The presented findings will contribute to clarify the fate and role of the SGs after C. erythraea ingestion within in vivo systems, and to further promote this remarkable plant as a food preservation additive with significant health benefits.
Ključne reči:
Centaurium erythraea; Metabolomics; Secoiridoid glucosides; Aglycones; Antioxidant activity; Antimicrobial activityIzvor:
Industrial Crops and Products, 2018, 111, 705-721Finansiranje / projekti:
- Fiziološka, hemijska i molekularna analiza diverziteta odabranih retkih i ugroženih biljnih vrsta u cilju ex situ zaštite i produkcije biološki aktivnih jedinjenja (RS-MESTD-Basic Research (BR or ON)-173024)
- Karakterizacija i primena metabolita gljiva i utvrđivanje potencijala novih biofungicida (RS-MESTD-Basic Research (BR or ON)-173032)
- Korelacija strukture i osobina prirodnih i sintetičkih molekula i njihovih kompleksa sa metalima (RS-MESTD-Basic Research (BR or ON)-172017)
DOI: 10.1016/j.indcrop.2017.11.040
ISSN: 0926-6690
WoS: 000419407600085
Scopus: 2-s2.0-85035801297
URI
http://linkinghub.elsevier.com/retrieve/pii/S0926669017307999https://radar.ibiss.bg.ac.rs/handle/123456789/2937