Genistein and daidzein treatments differently affect uterine homeostasis in the ovary-intact middle-aged rats.
2018
Аутори:
Jarić, IvanaŽivanović, Jasmina
Miler, Marko
Ajdžanović, Vladimir
Blagojević, Duško
Ristić, Nataša
Milošević, Verica
Nestorović, Nataša
Тип документа:
Чланак у часопису (Објављена верзија)
,
© 2017 Elsevier Inc
Метаподаци
Приказ свих података о документуАпстракт:
This study aimed to investigate the effects of soy isoflavones, genistein (GEN) and daidzein, (DAI) on the uterine function in ovary-intact middle-aged rats. GEN and DAI (35mg/kg) were subcutaneously administrated to acyclic (12-month-old) Wistar females, daily, for 4weeks. Control group received either vehicle (olive oil and ethanol, 9:1) or remained intact. We found that GEN and DAI differently affect uterine morphophysiology. GEN significantly increased the uterine wet weight which was associated with hyperplastic changes, revealed by stereological and histomorphometrical analyses. Also, PCNA immunoexpression was increased, whereas expression of apoptotic marker (caspase-3) was decreased. Protein and gene expressions of ERα were down-regulated, while PR and ERβ were up-regulated after GEN application. Also, GEN caused an increase of LAC and VEGF mRNA expression, together with an up-regulation of Akt activity. In contrast, DAI did not change the uterine wet weight and stereological features of the main uterine compartments as well as LAC and VEGF gene expression. Absence of hyperplastic changes were illustrated by an increase in caspase-3 immunoexpression, associated with reduced PCNA expression. DAI up-regulated only the expression of ERβ, while the expression levels of ERα and PR remain unaffected. Also, DAI inhibited the activation of Akt due to down-regulation of phosphorylated and total form of Akt protein expression. Compared to GEN, DAI did not promote events associated with the endometrial cell proliferation in the conducted study, figuring as the compound with a potential safety profile, which justifies further investigation.
Кључне речи:
Isoflavones; Middle-aged rats; Ovary-intact; Proliferation; Steroid receptors; Uterine functionИзвор:
Toxicology and Applied Pharmacology, 2018, 339, 73-84Финансирање / пројекти:
- COST Action FA 1403 POSITIVe (Interindividual variation in response to consumption of plant food bioactives and determinants involved)
- Одговор неуроендокриног система пацова на одабране биљне екстракте, фитоестрогене, стероидне и пептидне хормоне (RS-MESTD-Basic Research (BR or ON)-173009)
DOI: 10.1016/j.taap.2017.12.001
ISSN: 0041-008X
PubMed: 29217487
WoS: 000423781600008
Scopus: 2-s2.0-85037620773
URI
http://linkinghub.elsevier.com/retrieve/pii/S0041008X1730474Xhttp://www.ncbi.nlm.nih.gov/pubmed/29217487
https://radar.ibiss.bg.ac.rs/handle/123456789/2942