Delivery of [Ru(η6-p-cymene)Cl2{Ph2P(CH2)3SPh-κP}] using unfunctionalized and mercapto functionalized SBA-15 mesoporous silica: Preparation, characterization and in vitro study.
2018
Аутори:
Edeler, DavidArlt, Sören
Petković, Vladana
Ludwig, Gerd
Drača, Dijana
Maksimović-Ivanić, Danijela
Mijatović, Sanja
Kaluđerović, Goran N.
Тип документа:
Чланак у часопису (Објављена верзија)
,
© 2017 Elsevier Inc.
Метаподаци
Приказ свих података о документуАпстракт:
SBA-15 (Santa Barbara Amorphous 15) mesoporous silica and its functionalized form (with 3-mercaptopropyltriethoxysilane) SBA-15~SH were used as carriers for [Ru(η6-p-cymene)Cl2{Ph2P(CH2)3SPh-κP}] complex, denoted as [Ru]. Prepared mesoporous silica nanomaterials were characterized by traditional methods. Materials without [Ru] complex did not show any cytotoxic activity against melanoma B16 and B16-F10 cell lines. On the contrary, materials containing [Ru] such as SBA-15|[Ru] and SBA-15~SH|[Ru], exhibited very high activity against tested tumor cell lines, moreover with similar inhibitory potential. According to the loaded amount of the [Ru] in SBA-15|[Ru] and SBA-15~SH|[Ru] the IC50 values are 1-2μM depending on the test used, thus in comparison to [Ru] alone the activity of nanomaterials containing [Ru] are elevated 3-6 times in vitro. However, the mechanism of apoptosis induction differs for these two mesoporous silica. Unlike reference [Ru] compound and SBA-15~SH|[Ru], SBA-15|[Ru] induces high caspase activation. Discrepancy in mechanism of drugs action at intracellular level points towards an influence of functionalization as well as availability of the drug. Moreover, both SBA-15|[Ru] and SBA-15~SH|[Ru] similarly to [Ru] are declining autophagy in B16 cell line.
Кључне речи:
Apoptosis; Autophagy; Cytotoxicity; Drug delivery; Ruthenium(II) complex; SBA-15Извор:
Journal of Inorganic Biochemistry, 2018, 180, 155-162Финансирање / пројекти:
- Молекуларни механизми физиолошке и фармаколошке контроле инфламације и канцера (RS-MESTD-Basic Research (BR or ON)-173013)
DOI: 10.1016/j.jinorgbio.2017.12.011
ISSN: 0162-0134
PubMed: 29288894
WoS: 000426621000017
Scopus: 2-s2.0-85039450859
URI
http://linkinghub.elsevier.com/retrieve/pii/S0162013417306165http://www.ncbi.nlm.nih.gov/pubmed/29288894
https://radar.ibiss.bg.ac.rs/handle/123456789/2954