In Situ Detection of Programmed Cell Death in Senescing Nicotiana tabacum Leaves Using TUNEL Assay
2018
Тип документа:
Поглавље у монографији (Објављена верзија)
,
© Springer Science+Business Media, LLC 2018
Метаподаци
Приказ свих података о документуАпстракт:
Leaf senescence constitutes a highly regulated final phase of leaf development, leading to cell death that is recognized as a type of programmed cell death (PCD). Degradation of nuclear DNA into oligonucleosomal fragments (DNA ladder) and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay are methods commonly used to detect PCD-specific DNA cleavage. TUNEL reaction in situ labels free 3′-OH DNA strand breaks (nicks), thus allowing histological localization of nuclear DNA degradation during PCD. Here we describe in situ labeling of PCD-specific nuclear DNA fragmentation on conventional histological sections of senescing tobacco leaves. Incorporation of fluorescein-labeled dUTPs is detected by fluorescence microscopy, which enables in situ visualization of PCD at the single-cell level in the leaf mesophyll tissues undergoing senescence.
Кључне речи:
DNA fragmentation; Histology; Leaf senescence; Nicotiana tabacum; Programmed cell death; TUNEL assayУ:
- Guo Y, editor. Plant Senescence: Methods and Protocols. New York, NY: Humana Press; 2018. p. 267–82. (Methods in Molecular Biology; Vol. 1744)
DOI: 10.1007/978-1-4939-7672-0_21
ISBN: 978-1-4939-7672-0
PubMed: 29392672
Scopus: 2-s2.0-85041735150
URI
http://link.springer.com/10.1007/978-1-4939-7672-0_21https://radar.ibiss.bg.ac.rs/handle/123456789/3000