Differences in bioactivity of three endemic Nepeta species arising from main terpenoid and phenolic constituents
2018
Authors:
Nestorović Živković, JasminaŽivković, Suzana
Šiler, Branislav
Aničić, Neda
Dmitrović, Slavica
Divac-Rankov, Aleksandra
Giba, Zlatko
Mišić, Danijela
Document Type:
Article (Published version)
Metadata
Show full item recordAbstract:
Methanol extracts of three endemic Nepeta species were analyzed for their main secondary metabolites, terpenes and phenolics, and further investigated for antioxidant capacity and embryonic toxicity in zebrafish. UHPLC/DAD/(±) HESI-MS/MS analysis showed that the dominant compound in N. rtanjensis was trans,cis-nepetalactone, the cis,trans isomer of this monoterpene lactone was dominant in N. sibirica, while nepetalactone was detected only in traces in N. nervosa. In all investigated species, rosmarinic acid was the dominant phenolic compound, while other identified phenolic acids (chlorogenic, neochlorogenic and caffeic) were present in considerably lower amounts. ABTS and DPPH assays showed that the methanol extracts of N. rtanjensis, N. sibirica and especially N. nervosa possessed strong antioxidant activities, with the FRAP assay revealing high ferric-reducing abilities for all three tested species. Such a strong antioxidant potential, especially as manifested in the DPPH and FRAP assays, can be attributed to phenolic acids, and in the first place to rosmarinic acid. Increased lethality of zebrafish embryos in any of the treatments was not observed, but several toxic effects on embryonic development were recorded, such as pericardial and yolk sac edema. As in other Nepeta species, the three studied endemic species possessed a great potential for food conservation or as medicinal supplements if applied in optimized concentrations; however, alternative sources of plant material (e.g. field cultivation) should be established bearing in mind their vulnerability in nature.
Keywords:
Nepeta rtanjensis; Nepeta sibirica; Nepeta nervosa; Nepetalactone; Phenolic acidsSource:
Archives of Biological Sciences, 2018, 70, 1, 63-76Funding / projects:
- Physiological, chemical and molecular analysis of the diversity of selected rare and endangered plant species and application of biotechnology for ex situ conservation and production of biologically active compounds (RS-MESTD-Basic Research (BR or ON)-173024)
- Complex diseases as a model system for phenotype modulation- structural and functional analysis of molecular biomarkers (RS-MESTD-Basic Research (BR or ON)-173008)
DOI: 10.2298/ABS170616026N
ISSN: 0354-4664; 0354-4664
WoS: 000428370100007
Scopus: 2-s2.0-85043682981
URI
http://www.doiserbia.nb.rs/Article.aspx?ID=0354-46641700026Nhttps://radar.ibiss.bg.ac.rs/handle/123456789/3017
http://www.serbiosoc.org.rs/arch/index.php/abs/article/view/1881