The Role of Macrophage Migration Inhibitory Factor in the Function of Intestinal Barrier.
2018
Autori:
Vujičić, MilicaSaksida, Tamara
Despotović, Sanja
Soković Bajić, Svetlana
Lalić, Ivana
Koprivica, Ivan
Mićanović, Dragica
Golić, Natasa
Tolinački, Maja
Stojanović, Ivana D.
Tip dokumenta:
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt:
Macrophage migration inhibitory factor (MIF) is a multifunctional protein that is involved in the development of gut-related inflammation. To investigate the role of MIF in the function of the intestinal barrier, we have explored intestinal permeability and gut-associated immune response in MIF-deficient (MIF-KO) mice. The absence of MIF provoked impairment of tight and adherens epithelial junctions in the colon through the disturbance of E-cadherin, zonula occludens-1, occludin and claudin-2 expression, which lead to the increase of intestinal barrier permeability. In these circumstances the diversity and content of gut microbiota in MIF-KO mice was considerably different compared to wild type mice. This change in microbiota was accompanied by an increased intestinal IgA concentration and a higher production of pro-inflammatory cytokines TNF and IFN-γ in mesenteric lymph nodes of MIF-KO mice. The forced changes of microbiota executed by antibiotics prevented the "leakage" of the barrier in MIF-KO mice, probably through up-regulation of occludin expression and normalization of cellular pore diameters. In addition, cytokine secretion was normalized after the treatment with antibiotics. These results suggest that MIF participates in the maintenance of physiological microbiota diversity and immunosurveillance, which in turn enables the proper intestinal barrier function.
Izvor:
Scientific Reports, 2018, 8, 1, 6337-Finansiranje / projekti:
- Molekularni mehanizmi fiziološke i farmakološke kontrole inflamacije i kancera (RS-MESTD-Basic Research (BR or ON)-173013)
DOI: 10.1038/s41598-018-24706-3
PubMed: 29679061
WoS: 000430537800014
Scopus: 2-s2.0-85045886334
URI
http://www.nature.com/articles/s41598-018-24706-3http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC5910418
https://radar.ibiss.bg.ac.rs/handle/123456789/3049