Gut Microbiota Confers Resistance of Albino Oxford Rats to the Induction of Experimental Autoimmune Encephalomyelitis.
2018
Authors:
Stanisavljević, SuzanaDinić, Miroslav
Jevtić, Bojan
Nikolovski, Neda
Momčilović, Miljana
Đokić, Jelena
Golić, Nataša
Mostarica Stojković, Marija
Miljković, Đorđe
Document Type:
Article (Published version)
,
© 2018 Stanisavljević, Dinić, Jevtić, Đedović, Momčilović, Đokić, Golić, Mostarica Stojković and Miljković.
Metadata
Show full item recordAbstract:
Albino Oxford (AO) rats are extremely resistant to induction of experimental autoimmune encephalomyelitis (EAE). EAE is an animal model of multiple sclerosis, a chronic inflammatory disease of the central nervous system (CNS), with established autoimmune pathogenesis. The autoimmune response against the antigens of the CNS is initiated in the peripheral lymphoid tissues after immunization of AO rats with CNS antigens. Subsequently, limited infiltration of the CNS occurs, yet without clinical sequels. It has recently become increasingly appreciated that gut-associated lymphoid tissues (GALT) and gut microbiota play an important role in regulation and propagation of encephalitogenic immune response. Therefore, modulation of AO gut microbiota by antibiotics was performed in this study. The treatment altered composition of gut microbiota in AO rats and led to a reduction in the proportion of regulatory T cells in Peyer's patches, mesenteric lymph nodes, and in lymph nodes draining the site of immunization. Upregulation of interferon-γ and interleukin (IL)-17 production was observed in the draining lymph nodes. The treatment led to clinically manifested EAE in AO rats with more numerous infiltrates and higher production of IL-17 observed in the CNS. Importantly, transfer of AO gut microbiota into EAE-prone Dark Agouti rats ameliorated the disease. These results clearly imply that gut microbiota is an important factor in AO rat resistance to EAE and that gut microbiota transfer is an efficacious way to treat CNS autoimmunity. These findings also support the idea that gut microbiota modulation has a potential as a future treatment of multiple sclerosis.
Keywords:
Antibiotics; Experimental autoimmune encephalomyelitis; Gut microbiota; Gut microbiota transfer; Gut-associated lymphoid tissue; Multiple sclerosisSource:
Frontiers in Immunology, 2018, 9, 942-Funding / projects:
- Cellular and molecular mechanisms of recovery of rats from experimental autoimmune encephalomyelitis (RS-MESTD-Basic Research (BR or ON)-173035)
- Genes and molecular mechanisms promoting probiotic activity of lactic acid bacteria from Western Balkan (RS-MESTD-Basic Research (BR or ON)-173019)
- Immunopathogenic and regulatory mechanisms in autoimmune diseases and chronic inflamation (RS-MESTD-Basic Research (BR or ON)-175038)
- Molecular mechanisms of physiological and pharmacological control of inflammation and cancer (RS-MESTD-Basic Research (BR or ON)-173013)
DOI: 10.3389/fimmu.2018.00942
PubMed: 29770137
WoS: 000431197600001
Scopus: 2-s2.0-85046279847
URI
http://journal.frontiersin.org/article/10.3389/fimmu.2018.00942/fullhttp://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC5942155
https://radar.ibiss.bg.ac.rs/handle/123456789/3056