Involvement of glucocorticoid prereceptor metabolism and signaling in rat visceral adipose tissue lipid metabolism after chronic stress combined with high-fructose diet.
2018
Аутори:
Bursać, BiljanaĐorđević, Ana
Veličković, Nataša
Vojnović-Milutinović, Danijela
Petrović, Snježana
Teofilović, Ana
Gligorovska, Ljupka
Preitner, Frederic
Tappy, Luc
Matić, Gordana
Тип документа:
Чланак у часопису (Објављена верзија)
,
© 2018 Elsevier B.V.
Метаподаци
Приказ свих података о документуАпстракт:
Both fructose overconsumption and increased glucocorticoids secondary to chronic stress may contribute to overall dyslipidemia. In this study we specifically assessed the effects and interactions of dietary fructose and chronic stress on lipid metabolism in the visceral adipose tissue (VAT) of male Wistar rats. We analyzed the effects of 9-week 20% high fructose diet and 4-week chronic unpredictable stress, separately and in combination, on VAT histology, glucocorticoid prereceptor metabolism, glucocorticoid receptor subcellular redistribution and expression of major metabolic genes. Blood triglycerides and fatty acid composition were also measured to assess hepatic Δ9 desaturase activity. The results showed that fructose diet increased blood triglycerides and Δ9 desaturase activity. On the other hand, stress led to corticosterone elevation, glucocorticoid receptor activation and decrease in adipocyte size, while phosphoenolpyruvate carboxykinase, adipose tissue triglyceride lipase, FAT/CD36 and sterol regulatory element binding protein-1c (SREBP-1c) were increased, pointing to VAT lipolysis and glyceroneogenesis. The combination of stress and fructose diet was associated with marked stimulation of fatty acid synthase and acetyl-CoA carboxylase mRNA level and with increased 11β-hydroxysteroid dehydrogenase type 1 and hexose-6-phosphate dehydrogenase protein levels, suggesting a coordinated increase in hexose monophosphate shunt and de novo lipogenesis. It however did not influence the level of peroxisome proliferator-activated receptor-gamma, SREBP-1c and carbohydrate responsive element-binding protein. In conclusion, our results showed that only combination of dietary fructose and stress increase glucocorticoid prereceptor metabolism and stimulates lipogenic enzyme expression suggesting that interaction between stress and fructose may be instrumental in promoting VAT expansion and dysfunction.
Кључне речи:
Chronic unpredictable stress; Fructose; Glucocorticoids; Lipid metabolism; Visceral adipose tissueИзвор:
Molecular and Cellular Endocrinology, 2018Финансирање / пројекти:
- Улога стероидних хормона у неуроендокриној адаптацији на стрес и патофизиологији метаболичког синдрома - молекуларни механизми и клиничке импликације (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41009)
- SCOPES JRP (Grant No IZ73ZO_152331)
DOI: 10.1016/j.mce.2018.04.015
PubMed: 29729371
WoS: 000447986100013
Scopus: 2-s2.0-85046670211
URI
https://www.sciencedirect.com/science/article/pii/S0303720718301345?via%3Dihubhttp://www.ncbi.nlm.nih.gov/pubmed/29729371
https://radar.ibiss.bg.ac.rs/handle/123456789/3060