Drug Delivery System for Emodin Based on Mesoporous Silica SBA-15.
2018
Аутори:
Krajnović, TamaraMaksimović-Ivanić, Danijela
Mijatović, Sanja
Drača, Dijana
Wolf, Katharina
Edeler, David
Wessjohann, Ludger A
Kaluđerović, Goran N
Тип документа:
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт:
In this study mesoporous silica SBA-15 was evaluated as a vehicle for the transport of cytotoxic natural product emodin (EO). SBA-15 was loaded with different quantities of EO (SBA-15|EO1⁻SBA-15|EO5: 8⁻36%) and characterized by traditional methods. Several parameters (stabilities) and the in vitro behavior on tumor cell lines (melanoma A375, B16 and B16F10) were investigated. SBA-15 suppresses EO release in extremely acidic milieu, pointing out that EO will not be discharged in the stomach. Furthermore, SBA-15 protects EO from photodecomposition. In vitro studies showed a dose dependent decrease of cellular viability which is directly correlated with an increasing amount of EO in SBA-15 for up to 27% of EO, while a constant activity for 32% and 36% of EO in SBA-15 was observed. Additionally, SBA-15 loaded with EO (SBA-15|EO3) does not disturb viability of peritoneal macrophages. SBA-15|EO3 causes inhibition of tumor cell proliferation and triggers apoptosis, connected with caspase activation, upregulation of Bax, as well as Bcl-2 and Bim downregulation along with amplification of poly-(ADP-ribose)-polymerase (PARP) cleavage fragment. Thus, the mesoporous SBA-15 is a promising carrier of the water-insoluble drug emodin.
Кључне речи:
SBA-15; Apoptosis; Autophagy; Emodin; MelanomaИзвор:
Nanomaterials (Basel, Switzerland), 2018, 8, 5, 322-Финансирање / пројекти:
- Молекуларни механизми физиолошке и фармаколошке контроле инфламације и канцера (RS-MESTD-Basic Research (BR or ON)-173013)
DOI: 10.3390/nano8050322
PubMed: 29757198
WoS: 000435198300053
Scopus: 2-s2.0-85047644167
URI
http://www.mdpi.com/2079-4991/8/5/322http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC5977336
https://radar.ibiss.bg.ac.rs/handle/123456789/3078