Oxidative cost of interspecific hybridization: a case study of two Triturus species and their hybrids.
2018
Authors:
Prokić, MarkoDespotović, Svetlana
Vučić, Tijana
Petrović, Tamara
Gavrić, Jelena
Gavrilović, Branka
Radovanović, Tijana
Saičić, Zorica
Document Type:
Article (Accepted Version)
,
© 2018. Published by The Company of Biologists Ltd
Metadata
Show full item recordAbstract:
Oxidative stress has most recently been suggested as one of possible mechanisms responsible for reduced fitness of hybrids. To explore possible oxidative cost of hybridization, we examined antioxidant defense system parameters (superoxide dismutase, catalase, glutathione peroxidase, glutathione s-transferase, glutathione reductase, glutathione, SH groups), their interconnectedness (index of integration), and levels of oxidative damage (concentrations of lipid peroxides-TBARS) in lab-reared newt species, Triturus macedonicus and T. ivanbureschi, and their hybrid. Our results showed that parental species differed in antioxidant defense system parameters, but not in the levels of integration of the whole system and oxidative damage. Individuals of T. ivanbureschi had higher activities of superoxide dismutase, glutathione s-transferase and concentrations of glutathione. Hybrid individuals of crested newts displayed higher levels of the antioxidant defense system (higher superoxide dismutase, catalase, glutathione peroxidase activities and concentrations of SH groups), and a lower overall correlation of antioxidant system (lower index of integration) in comparison to both parental species, suggesting that they may possess a less efficient antioxidant defense system and a higher investment in maintaining oxidative balance. The higher investment in the antioxidant system could divert limited resources away from other functions and affect further hybrid fitness. The presented findings contribute to a better understanding of the antioxidant defense system of crested newts and their interspecies differences, and support the hypothesis that oxidative stress is one of the costs of interspecific hybridization.
Keywords:
Antioxidative defense system; Crested newts; Interspecific hybridization; Oxidative stress; TriturusSource:
The Journal of Experimental Biology, 2018Funding / projects:
- Molecular and physiological biomonitoring of aerobic organisms based on the determination of biochemical biomarkers of oxidative stress (RS-MESTD-Basic Research (BR or ON)-173041)
- Diversity of the amphibians and reptiles on the Balkan Peninsula: evolutionary and conservation aspects (RS-MESTD-Basic Research (BR or ON)-173043)
DOI: 10.1242/jeb.182055
PubMed: 30127083
WoS: 000447340900012
Scopus: 2-s2.0-85054458075
URI
http://jeb.biologists.org/content/early/2018/08/17/jeb.182055https://radar.ibiss.bg.ac.rs/handle/123456789/3151