Effects of Vitamin D3 on the NADPH Oxidase and Matrix Metalloproteinase 9 in an Animal Model of Global Cerebral Ischemia.
2018
Authors:
Velimirović, MilicaJevtić Dožudić, Gordana
Selaković, Vesna
Stojković, Tihomir
Puškaš, Nela
Zaletel, Ivan
Živković, Milica
Dragutinović, Vesna
Nikolić, Tatjana
Jelenković, Ankica
Đorović, Đorđe
Mirčić, Aleksandar
Petronijević, Nataša D.
Document Type:
Article (Published version)
Metadata
Show full item recordAbstract:
Decreased blood flow in the brain leads to a rapid increase in reactive oxygen species (ROS). NADPH oxidase (NOX) is an enzyme family that has the physiological function to produce ROS. NOX2 and NOX4 overexpression is associated with aggravated ischemic injury, while NOX2/4-deficient mice had reduced stroke size. Dysregulation of matrix metalloproteinases (MMPs) contributes to tissue damage. The active form of vitamin D3 expresses neuroprotective, immunomodulatory, and anti-inflammatory effects in the CNS. The present study examines the effects of the vitamin D3 pretreatment on the oxidative stress parameters and the expression of NOX subunits, MMP9, microglial marker Iba1, and vitamin D receptor (VDR), in the cortex and hippocampus of Mongolian gerbils subjected to ten minutes of global cerebral ischemia, followed by 24 hours of reperfusion. The ischemia/reperfusion procedure has induced oxidative stress, changes in the expression of NOX2 subunits and MMP9 in the brain, and increased MMP9 activity in the serum of experimental animals. Pretreatment with vitamin D3 was especially effective on NOX2 subunits, MMP9, and the level of malondialdehyde and superoxide anion. These results outline the significance of the NOX and MMP9 investigation in brain ischemia and the importance of adequate vitamin D supplementation in ameliorating the injury caused by I/R.
Source:
Oxidative medicine and cellular longevity, 2018, 2018, 3273654-Funding / projects:
- Structural and biochemical characteristics of synaptic plasticity alterations in psychiatric disorders (RS-MESTD-Basic Research (BR or ON)-175058)
- Antioxidative defense, differentiation and regeneration potential of tissue specific mesenchymal stem cells during ageing (RS-MESTD-Basic Research (BR or ON)-175061)
- University of Defence in Belgrade Medical Faculty of the Military Medical Academy (#MFVMA/11/16-18 “Cerebral ischemia - damaging mechanisms and neuroprotection”).
DOI: 10.1155/2018/3273654
ISSN: 1942-0900
PubMed: 29849881
WoS: 000431211100001
Scopus: 2-s2.0-85055075298
URI
https://www.hindawi.com/journals/omcl/2018/3273654/http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC5932460
https://radar.ibiss.bg.ac.rs/handle/123456789/3167