Identification of transcription factors involved in the transcriptional regulation of the CXCL12 gene in rat pancreatic insulinoma Rin-5F cell line
2015
Autori:
Marković, JelenaUskoković, Aleksandra
Grdović, Nevena
Dinić, Svetlana
Mihailović, Mirjana
Arambašić Jovanović, Jelena
Poznanović, Goran
Vidaković, Melita
Tip dokumenta:
Članak u časopisu (Objavljena verzija)
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© 2015 Published by NRC Research Press.
Metapodaci
Prikaz svih podataka o dokumentuApstrakt:
Diabetes is characterized by a deficit in the number of functional pancreatic β-cells. Understanding the mechanisms that stimulate neogenesis of β-cells should contribute to improved maintenance of β-cell mass. Chemokine CXCL12 has recently become established as a novel β-cell growth factor, however the mechanisms controlling its expression require clarification. We investigated the proteins involved in the transcriptional regulation of the rat β-cell CXCL12 gene (Cxcl12). Using the electrophoretic mobility shift assay and chromatin immunoprecipitation, we established the in vitro and in vivo binding of C/EBPβ, C/EBPα, STAT3, p53, FOXO3a, and HMG I/Y to the Cxcl12 promoter. Co-immunoprecipitation experiments revealed protein-protein interactions between YY1 and PARP-1, FOXO3a and PARP-1, Sp1 and PARP-1, p53 and PARP-1, C/EBPβ and PARP-1, YY1 and p53, YY1 and FOXO3a, p53 and FOXO3a, Sp1 and FOXO3a, C/EBPβ and FOXO3a, C/EBPα and FOXO3a, Sp1 and STAT3. Our data lay the foundation for research into the interplay of signaling pathways that determine the β-cell Cxcl12 expression profile.
Ključne reči:
CXCL12; CXCL12 gene promoter; Protein-protein interaction; Transcription factor-promoter interaction; Transcriptional regulationIzvor:
Biochemistry and cell biology, 2015, 93, 1, 54-62Finansiranje / projekti:
- Signalni molekuli u dijabetesu: identifikacija potencijalnih bioloških markera uključenih u modifikaciju i integraciju signalnih puteva u cilju predikcije i intervencije u dijabetesu (RS-MESTD-Basic Research (BR or ON)-173020)
DOI: 10.1139/bcb-2014-0104
ISSN: 0829-8211
PubMed: 25453873