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Identification of transcription factors involved in the transcriptional regulation of the CXCL12 gene in rat pancreatic insulinoma Rin-5F cell line
dc.creator | Marković, Jelena | |
dc.creator | Uskoković, Aleksandra | |
dc.creator | Grdović, Nevena | |
dc.creator | Dinić, Svetlana | |
dc.creator | Mihailović, Mirjana | |
dc.creator | Arambašić Jovanović, Jelena | |
dc.creator | Poznanović, Goran | |
dc.creator | Vidaković, Melita | |
dc.date.accessioned | 2018-11-15T09:31:49Z | |
dc.date.available | 2900-01-01 | |
dc.date.issued | 2015 | |
dc.identifier.issn | 0829-8211 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/pubmed/25453873 | |
dc.identifier.uri | http://www.scopus.com/inward/record.url?eid=2-s2.0-84921956361&partnerID=tZOtx3y1 | |
dc.identifier.uri | https://radar.ibiss.bg.ac.rs/handle/123456789/3176 | |
dc.description.abstract | Diabetes is characterized by a deficit in the number of functional pancreatic β-cells. Understanding the mechanisms that stimulate neogenesis of β-cells should contribute to improved maintenance of β-cell mass. Chemokine CXCL12 has recently become established as a novel β-cell growth factor, however the mechanisms controlling its expression require clarification. We investigated the proteins involved in the transcriptional regulation of the rat β-cell CXCL12 gene (Cxcl12). Using the electrophoretic mobility shift assay and chromatin immunoprecipitation, we established the in vitro and in vivo binding of C/EBPβ, C/EBPα, STAT3, p53, FOXO3a, and HMG I/Y to the Cxcl12 promoter. Co-immunoprecipitation experiments revealed protein-protein interactions between YY1 and PARP-1, FOXO3a and PARP-1, Sp1 and PARP-1, p53 and PARP-1, C/EBPβ and PARP-1, YY1 and p53, YY1 and FOXO3a, p53 and FOXO3a, Sp1 and FOXO3a, C/EBPβ and FOXO3a, C/EBPα and FOXO3a, Sp1 and STAT3. Our data lay the foundation for research into the interplay of signaling pathways that determine the β-cell Cxcl12 expression profile. | en |
dc.publisher | National Research Council of Canada | |
dc.publisher | Ottawa: Canadian Science Publishing | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173020/RS// | |
dc.rights | restrictedAccess | |
dc.source | Biochemistry and cell biology | |
dc.subject | CXCL12 | |
dc.subject | CXCL12 gene promoter | |
dc.subject | Protein-protein interaction | |
dc.subject | Transcription factor-promoter interaction | |
dc.subject | Transcriptional regulation | |
dc.title | Identification of transcription factors involved in the transcriptional regulation of the CXCL12 gene in rat pancreatic insulinoma Rin-5F cell line | en |
dc.type | article | en |
dc.rights.license | ARR | |
dcterms.abstract | Aрамбашић Јовановић, Јелена; Ускоковић, Aлександра; Грдовић, Невена; Динић, Светлана; Михаиловић, Мирјана; Познановић, Горан; Видаковић, Мелита; Марковић, Јелена; | |
dc.rights.holder | © 2015 Published by NRC Research Press. | |
dc.citation.issue | 1 | |
dc.citation.volume | 93 | |
dc.identifier.doi | 10.1139/bcb-2014-0104 | |
dc.identifier.pmid | 25453873 | |
dc.identifier.scopus | 2-s2.0-84921956361 | |
dc.identifier.wos | 000349022600007 | |
dc.citation.spage | 54 | |
dc.citation.epage | 62 | |
dc.type.version | publishedVersion | en |
dc.citation.rank | M23 |