The protective effect of a mix of Lactarius deterrimus and Castanea sativa extracts on streptozotocin-induced oxidative stress and pancreatic β-cell death
2012
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Аутори:
Grdović, NevenaDinić, Svetlana
Arambašić Jovanović, Jelena
Mihailović, Mirjana
Uskoković, Aleksandra
Marković, Jelena
Poznanović, Goran
Vidović, Senka
Zeković, Zoran
Mujić, Aida
Mujić, Ibrahim
Vidaković, Melita
Тип документа:
Чланак у часопису (Објављена верзија)
,
© 2011 The Authors.
Метаподаци
Приказ свих података о документуАпстракт:
Pancreatic β-cell death or dysfunction mediated by oxidative stress underlies the development and progression of diabetes mellitus. In the present study, we tested extracts from the edible mushroom Lactarius deterrimus and the chestnut Castanea sativa, as well as their mixture (MIX Ld/Cs), for potential beneficial effects on streptozotocin (STZ)-induced pancreatic β-cell death. Analysis of chelating effects, reducing power and radical-scavenging assays revealed strong antioxidant effects of the C. sativa extract and MIX Ld/Cs, while the L. deterrimus extract displayed a weak to moderate effect. The antioxidative effect of the chestnut extract corresponds with the high content of phenolics and flavonoids identified by HPLC analysis. In contrast, the mushroom extract contains relatively small amounts of phenols and flavonoids. However, both extracts, and especially their combination MIX Ld/Cs, increased cell viability after the STZ treatment as a result of a significant reduction of DNA damage and improved redox status. The chestnut extract and MIX Ld/Cs significantly lowered the STZ-induced increases in superoxide dismutase and catalase activities, while the mushroom extract had no impact on the activities of these antioxidant enzymes. However, the L. deterrimus extract exhibited good NO-scavenging activity. Different mechanisms that underlie antioxidant effects of the mushroom and chestnut extracts were discussed. When combined as in the MIX Ld/Cs, the extracts exhibited diverse but synergistic actions that ultimately exerted beneficial and protective effects against STZ-induced pancreatic β-cell death.
Кључне речи:
Antioxidant activity; Castanea sativa; Cytoprotection; Lactarius deterrimus; Rin-5F cellsИзвор:
The British Journal of Nutrition, 2012, 108, 7, 1163-1176Финансирање / пројекти:
- Сигнални молекули у дијабетесу: идентификација потенцијалних биолошких маркера укључених у модификацију и интеграцију сигналних путева у циљу предикције и интервенције у дијабетесу (RS-MESTD-Basic Research (BR or ON)-173020)
DOI: 10.1017/S0007114511006702
ISSN: 0007-1145
PubMed: 22186906
WoS: 000309947600004
Scopus: 2-s2.0-84869464304
URI
http://www.journals.cambridge.org/abstract_S0007114511006702https://radar.ibiss.bg.ac.rs/handle/123456789/3198