Tissue specific expression and genomic organization of bitter sesquiterpene lactone biosynthesis in Cichorium intybus L. (Asteraceae)
2019
Autori:
Bogdanović, MilicaCankar, Katarina
Todorović, Slađana
Dragićević, Milan
Simonović, Ana
van Houwelingen, Adele
Schijlen, Elio
Schipper, Bert
Gagneul, David
Hendriks, Theo
Quillet, Marie-Christine
Bouwmeester, Harro
Bosch, Dirk
Beekwilder, Jules
Tip dokumenta:
Članak u časopisu (Objavljena verzija)
,
© 2018 Elsevier B.V.
Metapodaci
Prikaz svih podataka o dokumentuApstrakt:
Chicory (Cichorium intybus L.) produces bitter sesquiterpene lactones (STLs). Some enzymes in the biosynthetic pathway towards these compounds have been characterized. However, the genomic organization and tissue specificity of their biosynthesis is largely unknown. Concentrations of two sesquiterpene lactones and expression of genes involved in the first dedicated biosynthetic step were measured in different chicory tissues. BAC clones containing different genes encoding germacrene A synthase were sequenced, and revealed several tightly linked paralogs. Promoters of genes encoding two germacrene A synthases were fused to GFP and expressed in plants regenerated from transformed chicory hairy root cultures. Highest expression was observed in the epidermis of leaves and external root tissue. This work opens the possibility to select for chicory germplasm diversified in STL content, and to study their role in chicory in defence and physiology.
Ključne reči:
Cichorium intybus; Sesquiterpene lactone; Genomic organisation; Gene expression; BiosynthesisIzvor:
Industrial Crops and Products, 2019, 129, 253-260Finansiranje / projekti:
- Plant Terpenoids for Human Health: a chemical and genomic approach to identify and produce bioactive compounds (EU-FP7-227448)
DOI: 10.1016/J.INDCROP.2018.12.011
WoS: 000457504200029
Scopus: 2-s2.0-85058158215
URI
https://www.sciencedirect.com/science/article/pii/S092666901831063X?via%3Dihubhttps://radar.ibiss.bg.ac.rs/handle/123456789/3212