Expression profiles of organogenesis-related genes over the time course of one-step de novo shoot organogenesis from intact seedlings of kohlrabi.
2019
Аутори:
Ćosić, TatjanaRaspor, Martin
Savić, Jelena
Cingel, Aleksandar
Matekalo, Dragana
Zdravković-Korać, Snežana
Ninković, Slavica
Тип документа:
Чланак у часопису (Објављена верзија)
,
© 2018 Elsevier GmbH
Метаподаци
Приказ свих података о документуАпстракт:
Kohlrabi (Brassica oleracea var. gongylodes) is an important vegetable crop that is able to undergo shoot regeneration in culture from intact seedlings in a single-step regeneration process, using cytokinin as the only plant growth regulator. In this work, we present the expression profiles of seven organogenesis-related genes over the time course of shoot regeneration from intact seedlings of kohlrabi cv. Vienna Purple on shoot regeneration media containing trans-zeatin, cis-zeatin, benzyl adenine or thidiazuron. Two auxin transporter genes - PIN3 and PIN4, a cytokinin response regulator - ARR5, two shoot apical meristem-related transcription factors - CUC1 and RGD3, and two cell cycle-related genes - CDKB2;1 and CYCB2;4 - displayed bimodal expression patterns on most cytokinin-containing media when their expression levels were normalized against control plants grown on hormone-free media. The first expression peak corresponded to direct upregulation by cytokinin from the growth media, and the second one reflected transcriptional events related to callus formation and/or acquisition of organogenic competence, corresponding to the shoot regeneration phases that have already been characterized in Arabidopsis thaliana. We demonstrate that the genes involved in the two-step shoot regeneration of Arabidopsis display their expected expression profiles during the single-step shoot regeneration of its close phylogenetic relative kohlrabi confirming the universality of their roles in the distinct phases of the regeneration process in Brassicaceae. The results presented here represent a first step towards genetic characterization of the morphogenetic processes in this important crop species.
Кључне речи:
Cytokinin; Gene expression; Kohlrabi; de novo shoot organogenesisИзвор:
Journal of Plant Physiology, 2019, 232, 257-269Финансирање / пројекти:
- Биотехнологија ин витро - гајене, лековите и угрожене биљне врсте (RS-MESTD-Basic Research (BR or ON)-173015)
- Физиолошка, хемијска и молекуларна анализа диверзитета одабраних ретких и угрожених биљних врста у циљу еx ситу заштите и продукције биолошки активних једињења (RS-MESTD-Basic Research (BR or ON)-173024)
DOI: 10.1016/j.jplph.2018.11.004
PubMed: 30537612
WoS: 000454881600029
Scopus: 2-s2.0-85057760427
URI
https://www.sciencedirect.com/science/article/pii/S0176161718304140?via%3Dihubhttps://radar.ibiss.bg.ac.rs/handle/123456789/3213