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Expression profiles of organogenesis-related genes over the time course of one-step de novo shoot organogenesis from intact seedlings of kohlrabi.
dc.creator | Ćosić, Tatjana | |
dc.creator | Raspor, Martin | |
dc.creator | Savić, Jelena | |
dc.creator | Cingel, Aleksandar | |
dc.creator | Matekalo, Dragana | |
dc.creator | Zdravković-Korać, Snežana | |
dc.creator | Ninković, Slavica | |
dc.date.accessioned | 2018-12-20T08:33:08Z | |
dc.date.available | 2900-01-01 | |
dc.date.issued | 2019 | |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S0176161718304140?via%3Dihub | |
dc.identifier.uri | https://radar.ibiss.bg.ac.rs/handle/123456789/3213 | |
dc.description.abstract | Kohlrabi (Brassica oleracea var. gongylodes) is an important vegetable crop that is able to undergo shoot regeneration in culture from intact seedlings in a single-step regeneration process, using cytokinin as the only plant growth regulator. In this work, we present the expression profiles of seven organogenesis-related genes over the time course of shoot regeneration from intact seedlings of kohlrabi cv. Vienna Purple on shoot regeneration media containing trans-zeatin, cis-zeatin, benzyl adenine or thidiazuron. Two auxin transporter genes - PIN3 and PIN4, a cytokinin response regulator - ARR5, two shoot apical meristem-related transcription factors - CUC1 and RGD3, and two cell cycle-related genes - CDKB2;1 and CYCB2;4 - displayed bimodal expression patterns on most cytokinin-containing media when their expression levels were normalized against control plants grown on hormone-free media. The first expression peak corresponded to direct upregulation by cytokinin from the growth media, and the second one reflected transcriptional events related to callus formation and/or acquisition of organogenic competence, corresponding to the shoot regeneration phases that have already been characterized in Arabidopsis thaliana. We demonstrate that the genes involved in the two-step shoot regeneration of Arabidopsis display their expected expression profiles during the single-step shoot regeneration of its close phylogenetic relative kohlrabi confirming the universality of their roles in the distinct phases of the regeneration process in Brassicaceae. The results presented here represent a first step towards genetic characterization of the morphogenetic processes in this important crop species. | en |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173015/RS// | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173024/RS// | |
dc.rights | restrictedAccess | |
dc.source | Journal of Plant Physiology | |
dc.subject | Cytokinin | |
dc.subject | Gene expression | |
dc.subject | Kohlrabi | |
dc.subject | de novo shoot organogenesis | |
dc.title | Expression profiles of organogenesis-related genes over the time course of one-step de novo shoot organogenesis from intact seedlings of kohlrabi. | en |
dc.type | article | en |
dc.rights.license | ARR | |
dcterms.abstract | Ћосић, Татјана; Цингел, Aлександар; Здравковић-Кораћ, Снежана; Распор, Мартин; Савић, Јелена; Матекало, Драгана; Нинковић, Славица; | |
dc.rights.holder | © 2018 Elsevier GmbH | |
dc.citation.volume | 232 | |
dc.identifier.doi | 10.1016/j.jplph.2018.11.004 | |
dc.identifier.pmid | 30537612 | |
dc.identifier.scopus | 2-s2.0-85057760427 | |
dc.identifier.wos | 000454881600029 | |
dc.citation.apa | Ćosić, T., Raspor, M., Savić, J., Cingel, A., Matekalo, D., Zdravković-Korać, S., & Ninković, S. (2018). Expression profiles of organogenesis-related genes over the time course of one-step de novo shoot organogenesis from intact seedlings of kohlrabi. Journal of Plant Physiology, 232, 257–269. | |
dc.citation.vancouver | Ćosić T, Raspor M, Savić J, Cingel A, Matekalo D, Zdravković-Korać S, Ninković S. Expression profiles of organogenesis-related genes over the time course of one-step de novo shoot organogenesis from intact seedlings of kohlrabi. J Plant Physiol. 2018;232:257–69. | |
dc.citation.spage | 257 | |
dc.citation.epage | 269 | |
dc.type.version | publishedVersion | en |
dc.citation.rank | M21 |