Aktivnost glutation-peroksidaze (GSH-Px), glutation-reduktaze (GR) i enzima faze II biotransformacije glutation-S-transferaze (GST) u tkivima nekih slatkovodnih rakova

Abstract

We investigated the activities of glutathione peroxidase (GSH-Px) glutathione reductase (GR) and phase II biotransformation enzyme glutathione-S-transferase (GST) in the hepatopancreas, the gills and muscle of Noble crayfish (Astacus astacus-AST), Stone crayfish (Austropotamobius torrentium-AUS) and Spynicheek crayfish (Orconectes limosus-ORC) from the South Morava River, Krajkovačka River and Serbian part of the Danube. Specific GSH-Px activity was lower in the hepatopancreas of AUS than in ORC and in the muscle of AST than in AUS and ORC. We obtained differences of total GSH-Px activity in the muscle between AST and AUS and between AST and ORC. Specific and total GR activity was higher in the hepatopancreas of AST than in other species. Specific and total activity of GR in the gills and in the muscle of crayfish species was higher in AUS then in AST and ORC. The specific and total GST activity was lower in the hepatopancreas of AST and ORC than in AUS. Specific and total GST activity in the gills was higher in AUS than in AST. The lowest specific and total GST activity was detected in the muscle of ORC compared to the AST and AUS. Our results show that specific and total enzyme activities of GSH-Px, GR and GST have species and tissue specifics between crayfish species and represent biomarkers of environmental influences on these organisms.

Određivali smo aktivnost glutation-peroksidaze (GSH-Px), glutation-reduktaze (GR) i enzima faze II biotransformacije glutation-S-transferaze (GST) u hepatopankreasu, škrgama i mišiću rečnog raka (Astacus astacus-AST), raka kamenjara (Austropotamobius torrentium-AUS) i američkog raka (Orconectes limosus-ORC) iz Južne Morave, Krajkovačke reke i Dunava. Specifična aktivnost GSH-Px je manja u hepatopankreasu kod AUS nego kod ORC, kao i u mišiću AST u poređenju sa AUS i ORC. Postoji razlika u aktivnosti između ukupne GSH-Px u mišiću kod AST i AUS, kao i između AST i ORC. Specifična i totalna aktivnost GR je veća u hepatopankreasu kod AST nego kod druge dve vrste, dok je u škrgama i mišiću rakova veća kod AUS nego kod AST i ORC. Specifična i totalna aktivnost GST je manja u hepatopankreasu kod AST i ORC u poređenju sa AUS, a u škrgama je veća kod AUS nego kod AST. Najmanja specifična i totalna GST aktivnost je u mišiću kod ORC u poređenju sa AST i AUS. Specifična i ukupna aktivnost GSH-Px, GR i GST pokazuje veliku specifičnost za vrstu i tkivo između ispitivanih vrsta rakova i predstavlja biomarker uticaja sredine na ove organizme.